[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.4014/jmb.1607.07022

Development of a Multiplex PCR for Discrimination of the TLC:RS1:CTX array of Vibrio cholerae Wave 3 El Tor Strains

Kim, Eun Jin (Department of Pharmacy, College of Pharmacy, Hanyang University)
Yu, Hyun Jin (Department of Pharmacy, College of Pharmacy, Hanyang University)
Nair, G. Balakrish (South East Asia Regional Office, World Health Organization)
Kim, Dong Wook (Department of Pharmacy, College of Pharmacy, Hanyang University)

Publication Information

Journal of Microbiology and Biotechnology / v.26, no.12, 2016 , pp. 2199-2205 More about this Journal

Abstract

Vibrio cholerae O1 serogroup Wave 3 El Tor strains are presently prevalent worldwide. The Wave 3 El Tor strains contain a TLC:RS1:CTX array on chromosome 1, and no element is integrated on chromosome 2. A multiplex PCR optimized to identify the TLC:RS1:CTX array of Wave 3 strains has been developed in this study. By using eight primers, the multiplex PCR can identify the characteristic CTX and RS1 array of Wave 3 strains from various arrays of strains belonging to other Waves. The four amplified DNA fragments of Wave 3 strains have been cloned in a vector, which could be used as a positive control for the multiplex PCR. This multiplex PCR and the positive control set could be useful tools for rapid recognition of Wave 3 El Tor strains.

Keywords

V. cholerae; multiplex PCR;

Citations & Related Records

Times Cited By KSCI : 1 (Citation Analysis)

Reference
Cited By KSCI

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