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http://dx.doi.org/10.4014/jmb.1605.05057

Isolation and Proteomic Analysis of a Chlamydomonas reinhardtii Mutant with Enhanced Lipid Production by the Gamma Irradiation Method  

Baek, Jaewon (Department of Biotechnology and Bioengineering, Interdisciplinary Program of Bioenergy and Biomaterials, Chonnam National University)
Choi, Jong-il (Department of Biotechnology and Bioengineering, Interdisciplinary Program of Bioenergy and Biomaterials, Chonnam National University)
Park, Hyun (Korea Polar Research Institute)
Lim, Sangyong (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
Park, Si Jae (Department of Environmental Engineering and Energy, Myongji University)
Publication Information
Journal of Microbiology and Biotechnology / v.26, no.12, 2016 , pp. 2066-2075 More about this Journal
Abstract
In this study, an enhanced lipid-producing mutant strain of the microalga Chlamydomonas reinhardtii was developed by gamma irradiation. To induce the mutation, C. reinhardtii was gamma irradiated at a dose of 400 Gy. After irradiation, the surviving cells were stained with Nile red. The mutant (Cr-4013) accumulating 20% more lipid than the wild type was selected. Thin-layer chromatography revealed the triglyceride and free fatty acid contents to be markedly increased in Cr-4013. The major fatty acids identified were palmitic acid, oleic acid, linoleic acid, and linolenic acid. Random amplified polymeric DNA analysis showed partial genetic modifications in Cr-4013. To ascertain the changes of protein expression in the mutant strain, two-dimensional electrophoresis was conducted. These results showed that gamma radiation could be used for the development of efficient microalgal strains for lipid production.
Keywords
Chlamydomonas reinhardtii; lipid content; mutant; proteome;
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