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http://dx.doi.org/10.4014/jmb.1502.02037

Properties of a Bacteriocin Produced by Bacillus subtilis EMD4 Isolated from Ganjang (Soy Sauce)  

Liu, Xiaoming (Division of Applied Life Science (BK21 Plus), Graduate School, Gyeongsang National University)
Lee, Jae Yong (Division of Applied Life Science (BK21 Plus), Graduate School, Gyeongsang National University)
Jeong, Seon-Ju (Division of Applied Life Science (BK21 Plus), Graduate School, Gyeongsang National University)
Cho, Kye Man (Department of Food Science, Gyeongnam National University of Science and Technology)
Kim, Gyoung Min (Namhae Garlic Research Institute)
Shin, Jung-Hye (Namhae Garlic Research Institute)
Kim, Jong-Sang (School of Food Science and Biotechnology (BK21 Plus), Kyungpook National University)
Kim, Jeong Hwan (Division of Applied Life Science (BK21 Plus), Graduate School, Gyeongsang National University)
Publication Information
Journal of Microbiology and Biotechnology / v.25, no.9, 2015 , pp. 1493-1501 More about this Journal
Abstract
A Bacillus species, EMD4, with strong antibacterial activity was isolated from ganjang (soy sauce) and identified as B. subtilis. B. subtilis EMD4 strongly inhibited the growth of B. cereus ATCC14579 and B. thuringiensis ATCC33679. The antibacterial activity was stable at pH 3-9 but inactive at pH 10 and above. The activity was fully retained after 15 min at 80℃ but reduced by 50% after 15 min at 90℃. The activity was completely destroyed by proteinase K and protease treatment, indicating its proteinaceous nature. The bacteriocin (BacEMD4) was partially purified from culture supernatant by ammonium sulfate precipitation, and Q-Sepharose and Sephadex G-50 column chromatographies. The specific activity was increased from 769.2 AU/mg protein to 8,347.8 AU/mg protein and the final yield was 12.6%. The size of BacEMD4 was determined to be 3.5 kDa by Tricine SDS-PAGE. The N-terminal amino acid sequence was similar with that of Subtilosin A. Nucleotide sequencing of the cloned gene confirmed that BacEMD4 was Subtilosin A. BacEMD4 showed bactericidal activity against B. cereus ATCC14579.
Keywords
Bacteriocin; Bacillus subtilis; antimicrobial activity; ganjang;
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