Browse > Article
http://dx.doi.org/10.4014/jmb.1412.12083

Promotion Effects of Ultra-High Molecular Weight Poly-γ-Glutamic Acid on Wound Healing  

Choi, Jae-Chul (BioLeaders CorporationDepartment of Bio and Fermentation Convergence Technology, Kookmin University)
Uyama, Hiroshi (Department of Applied Chemistry, Graduate School of Engineering, Osaka University)
Lee, Chul-Hoon (Department of Pharmacy, College of Pharmacy, Hanyang University)
Sung, Moon-Hee (BioLeaders Corporation)
Publication Information
Journal of Microbiology and Biotechnology / v.25, no.6, 2015 , pp. 941-945 More about this Journal
Abstract
We examined the in vivo efficacy of ultra-high molecular weight poly-γ-glutamic acid (UHMW γ-PGA) for wound healing. The wound area was measured by a ruler and documented by digital photography before the animals were sacrificed at days 8 and 16 post wounding. The areas of wounds treated with UHMW γ-PGA were significantly decreased on days 8 and 16, as compared with those receiving a control treatment, and more than 70% of the UHMW γ-PGAtreated area was repaired by day 8. Hematoxylin and eosin staining confirmed that the epidermis had regenerated in the UHMW γ-PGA-treated wounds. At 16 days post wounding, collagen pigmentation and cross-linking were increased as compared with the control groups, and greater regeneration of blood vessels had occurred in UHMW γ-PGA-treated groups. Increased levels of transforming growth factor-beta and β-catenin were also observed in skin samples collected from UHMW γ-PGA-treated animals on days 8 and 16 post incision. Taken together, these findings suggest that UHMW γ-PGA promotes wound healing in vivo.
Keywords
Ultra-high molecular weight poly-γ-glutamic acid (UHMW γ-PGA); wound healing;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Penn JW, Grobbelaar AO, Rolfe KJ. 2012. The role of the TGF-β family in wound healing, burns and scarring: a review. Int. J. Burns Trauma 2: 18-28.
2 Reish RG, Eriksson E. 2008. Scars: a review of emerging and currently available therapies. Plast. Reconstr. Surg. 122: 1068-1078.   DOI   ScienceOn
3 Silkstone D, Hong H, Alman BA. 2008. Beta-catenin in the race to fracture repair: in it to Wnt. Nat. Clin. Pract. Rheumatol. 4: 413-419.   DOI   ScienceOn
4 Singer AF, Clark RA. 1999. Cutaneous wound healing. N. Engl. J. Med. 341: 738-746.   DOI   ScienceOn
5 Tiede S, Ernst N, Bayat A, Paus R, Tronnier V, Zechel C. 2009. Basic fibroblast growth factor: a potential new therapeutic tool for the treatment of hypertrophic and keloid scars. Ann. Anat. 191: 33-44.   DOI   ScienceOn
6 Lee TY, Kim YH, Yoon SW, Choi JC, Yang JM, Kim CJ, et al. 2009. Oral administration of poly-gamma-glutamate induces TLR4- and dendritic cell-dependent antitumor effect. Cancer Immunol. Immunother. 58: 1781-1794.   DOI
7 Kim TW, Lee TY, Bae HC, Hahm JH, Kim YH, Park C, et al. 2007. Oral administration of high molecular mass poly-gammaglutamate induces NK cell-mediated antitumor immunity. J. Immunol. 179: 775-780.   DOI
8 Koivisto L, Hakkinen L, Larjava H. 2012. Re-epithelialization of wounds. Endodontic Topics 24: 59-93.   DOI   ScienceOn
9 Larson BJ, Longaker MT, Lorenz HP. 2010. Scarless fetal wound healing: a basic science review. Plast. Reconstr. Surg. 126: 1172-1180.   DOI   ScienceOn
10 Liu B, Lu X, Qi C, Zheng S, Zhou M, Wang J, Yin W. 2014. KGFR promotes Na+ channel expression in a rat acute lung injury model. Afr. Health Sci. 14: 648-656.   DOI
11 Longaker MT, Whitby DJ, Ferguson MW, Lotenz HP, Harroson MR, Adzick NS. 1994. Adult skin wounds in the fetal environment heal with scar formation. Ann. Surg. 219: 65-72.   DOI
12 Ma Y, Zhao H, Zhou X. 2002. Topical treatment with growth factors for tympanic membrane perforations: progress towards clinical application. Acta Otolaryngol. 122: 586-599.   DOI
13 Mehraein F, Sarbishegi M, Aslani A. 2014. Evaluation of effect of oleuropein on skin wound healing in aged male BALB/c mice. Cell J. 16: 25-30.
14 Park C, Sung MH. 2009. New bioindustrial development of high molecular weight of poly-gamma-glutamic acid produced by Bacillus subtilis (Chungkookjang). Polym. Sci. Technol. 20: 440-446.
15 Chrissouli S, Pratsinis H, Velissariou V, Anastasiou A, Kletsas D. 2010. Human amniotic fluid stimulates the proliferation of human fetal and adult skin fibroblasts: the roles of bFGF and PDGF and of the ERK and Akt signaling pathways. Wound Repair Regen. 18: 643-654.   DOI   ScienceOn
16 Adam ET, Tokarz D, Prent N, Cisek R, Alami J, Dumont DJ, et al. 2010. Nonlinear multicontrast microscopy of hematoxylinand-eosin-stained histological sections. J. Biomed. Opt. 15: 026018.   DOI   ScienceOn
17 Adzick NS, Lorenz HP. 1994. Cells, matrix, growth factors, and the surgeon. Ann. Surg. 220: 10-18.   DOI
18 Bae SR, Park C, Choi JC, Poo H, Kim CJ, Sung MH. 2010. Effects of ultra high molecular weight poly-g-glutamic acid from Bacillus subtilis (Chungkookjang) on corneal wound healing. J. Microbiol. Biotechnol. 20: 803-808.
19 Colwell AS, Phan TT, Kong W, Longaker MT, Lorenz PH. 2005. Hypertrophic scar fibroblasts have increased connective tissue growth factor expression after transforming growth factor-beta stimulation. Plast. Reconstr. Surg. 116: 1387-1390.   DOI   ScienceOn
20 Diegelmann RF, Evans MC. 2004. Wound healing: an overview of acute, fibrotic and delayed healing. Front. Biosci. 9: 283-289.   DOI
21 Haertel B, von Woedtke T, Weltmann KD, Lindequist U. 2014. Non-thermal atmospheric-pressure plasma possible application in wound healing. Biomol. Ther. 22: 477-490.   DOI   ScienceOn
22 Horobin RW 2002. Biological staining: mechanisms and theory. Biotech. Histochem. 77: 3-13.   DOI
23 Jonsson K, Jensen JA, Goodson WH, Scheuenstuhl H, West J, Hopf JW, Hunt TK. 1991. Tissue oxygenation, anemia, and perfusion in relation to wound healing in surgical patients. Ann. Surg. 214: 605-613.   DOI