Browse > Article
http://dx.doi.org/10.4014/jmb.1501.01081

Mitotic Cohesin Subunit Mcd1 Regulates the Progression of Meiotic Recombination in Budding Yeast  

Lee, Min-Su (Department of Life Science, Chung-Ang University)
Yoon, Sang-Wook (Department of Life Science, Chung-Ang University)
Kim, Keun Pil (Department of Life Science, Chung-Ang University)
Publication Information
Journal of Microbiology and Biotechnology / v.25, no.5, 2015 , pp. 598-605 More about this Journal
Abstract
The cohesin complex holds sister chromatids together and prevents premature chromosome segregation until the onset of anaphase. Mcd1 (also known as Scc1), the α-kleisin subunit of cohesin, is a key regulatory subunit of the mitotic cohesin complex and is required for maintaining sister chromatid cohesion, chromosome organization, and DNA repair. We investigated the function of Mcd1 in meiosis by ectopically expressing Mcd1 during early meiotic prophase I in Saccharomyces cerevisiae. Mcd1 partially regulated the progression of meiotic recombination, sister chromatid separation, and nuclear division. DNA physical analysis during meiotic recombination showed that Mcd1 induced double-strand breaks (DSBs) but negatively regulated homologous recombination during DSB repair; Mcd1 expression delayed post-DSB stages, leading to inefficiencies in the DSB-to-joint molecule (JM) transition and subsequent crossover formation. These findings indicate that meiotic cells undergo Mcd1-mediated DSB formation during prophase I, and that residual Mcd1 could regulate the progression of JM formation during meiotic recombination.
Keywords
Cohesin; Mcd1; double-strand break; homologous recombination;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Klein F, Mahr P, Galova M, Buonomo SB, Michaelis C, Nairz K, Nasmyth K. 1999. A central role for cohesins in sister chromatid cohesion, formation of axial elements, and recombination during yeast meiosis. Cell 98: 91-103.   DOI   ScienceOn
2 Krejci L, Altmannova V, Spirek M, Zhao X. 2012. Homologous recombination and its regulation. Nucleic Acids Res. 40: 5795-5818.   DOI
3 Kushnirov VV. 2000. Rapid and reliable protein extraction from yeast. Yeast 16: 857-860.   DOI
4 Nasmyth K, Peters JM, Uhlmann F. 2000. Splitting the chromosome: cutting the ties that bind sister chromatids. Science 288: 1379-1384.   DOI   ScienceOn
5 Brar GA, Kiburz BM, Zhang Y, Kim JE, White F, Amon A. 2006. Rec8 phosphorylation and recombination promote the step-wise loss of cohesins in meiosis. Nature 441: 532-536.   DOI   ScienceOn
6 Brar GA, Hochwagen A, Ee LS, Amon A. 2009. The multiple roles of cohesin in meiotic chromosome morphogenesis and pairing. Mol. Biol. Cell 20: 1030-1047.   DOI   ScienceOn
7 Guacci V, Yamamoto A, Strunnikov A, Kingsbury J, Hogan E, Meluh P, Koshland D. 1993. Structure and function of chromosomes in mitosis of budding yeast. Cold Spring Harb. Symp. Quant. Biol. 58: 677-685.   DOI
8 Guacci V, Koshland D, Strunnikov A. 1997. A direct link between sister chromatid cohesion and chromosome condensation revealed through the analysis of MCD1 in S. cerevisiae. Cell 91: 47-57.   DOI   ScienceOn
9 Hong S , Sung Y , Yu M, Lee M, Kleckner N, Kim KP. 2013. The logic and mechanism of homologous recombination partner choice. Mol. Cell 51: 440-453.   DOI   ScienceOn
10 Heidinger-Pauli JM, Unal E, Guacci V, Koshland D. 2008. The kleisin subunit of cohesin dictates damage-induced cohesion. Mol. Cell 31: 47-56.   DOI   ScienceOn
11 Hochwagen A, Amon A. 2006. Checking your breaks: surveillance mechanisms of meiotic recombination. Curr. Biol. 16: 217-228.   DOI   ScienceOn
12 Hong S, Kim KP. 2013. Shu1 promotes homolog bias of meiotic recombination in Saccharomyces cerevisiae. Mol. Cells 36: 446-454.   DOI
13 Hunter N, Kleckner N. 2001. The single-end invasion: an asymmetric intermediate at the double-strand break to doubleholliday junction transition of meiotic recombination. Cell 106: 59-70.   DOI   ScienceOn
14 Schwacha A, Kleckner N. 1995. Identification of double holliday junctions as intermediates in meiotic recombination. Cell 83: 783-791.   DOI   ScienceOn
15 Padmore R, Cao L, Kleckner N. 1991. Temporal comparison of recombination and synaptonemal complex formation during meiosis in S. cerevisiae. Cell 66: 1239-1256.   DOI   ScienceOn
16 San-Segundo PA, Roeder GS. 1999. Pch2 links chromatin silencing to meiotic checkpoint control. Cell 97: 313-324.   DOI   ScienceOn
17 Schwacha A, Kleckner N. 1994. Identification of joint molecules that form frequently between homologs but rarely between sister chromatids during yeast meiosis. Cell 76: 51-63.   DOI   ScienceOn
18 Sjögren C, Nasmyth K. 2001. Sister chromatid cohesion is required for postreplicative double-strand break repair in Saccharomyces cerevisiae. Curr. Biol. 11: 991-995.   DOI   ScienceOn
19 Yang H, Ren Q, Zhang Z. 2008. Cleavage of Mcd1 by caspase-like protease Esp1 promotes apoptosis in budding yeast. Mol. Biol. Cell 19: 2127-2134.   DOI   ScienceOn
20 Schwacha A, Kleckner N. 1997. Interhomolog bias during meiotic recombination: meiotic functions promote a highly differentiated interhomolog-only pathway. Cell 90: 1123-1135.   DOI   ScienceOn
21 Sym M, Engebrecht JA, Roeder GS. 1993. Zip1 is a synaptonemal complex protein required for meiotic chromosome synapsis. Cell 72: 365-378.   DOI   ScienceOn
22 Hunter N. 2006. Meiotic recombination, pp. 381-442. In Aguilera A, Rothstein R (eds.). Topics in Current Genetics, Molecular Genetics of Recombination. Springer-Verlag, Heidelberg.
23 Kadyk LC, Hartwell LH. 1992. Sister chromatids are preferred over homologs as substrates for recombinational repair in Saccharomyces cerevisiae. Genetics 132: 387-402.
24 Kateneva AV, Konovchenko AA, Guacci V, Dresser ME. 2005. Recombination protein Tid1p controls resolution of cohesin-dependent linkages in meiosis in Saccharomyces cerevisiae. J. Cell Biol. 171: 241-253   DOI   ScienceOn
25 Kim KP, Weiner BM, Zhang L, Jordan A, Dekker J, Kleckner N. 2010. Sister cohesion and structural axis components mediate homolog bias of meiotic recombination. Cell 143: 924-937.   DOI   ScienceOn
26 Molnar M, Bähler J, Sipiczki M, Kohli H. 1995. The rec8 gene of Schizosaccharomyces pombe is involved in linear element formation, chromosome pairing and sister-chromatid cohesion during meiosis. Genetics 141: 61-73.