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http://dx.doi.org/10.4014/jmb.1412.12007

Batch Conversion of Methane to Methanol Using Methylosinus trichosporium OB3b as Biocatalyst  

Hwang, In Yeub (Department of Chemical Engineering, Kyung Hee University)
Hur, Dong Hoon (Department of Chemical Engineering, Kyung Hee University)
Lee, Jae Hoon (Department of Chemical Engineering, Kyung Hee University)
Park, Chang-Ho (Department of Chemical Engineering, Kyung Hee University)
Chang, In Seop (School of Environmental Science and Engineering, Gwangju Institute of Science and Technology)
Lee, Jin Won (Department of Chemical and Biomolecular Engineering, Sogang University)
Lee, Eun Yeol (Department of Chemical Engineering, Kyung Hee University)
Publication Information
Journal of Microbiology and Biotechnology / v.25, no.3, 2015 , pp. 375-380 More about this Journal
Abstract
Recently, methane has attracted much attention as an alternative carbon feedstock since it is the major component of abundant shale and natural gas. In this work, we produced methanol from methane using whole cells of Methylosinus trichosporium OB3b as the biocatalyst. M. trichosporium OB3b was cultured on NMS medium with a supply of 7:3 air/methane ratio at 30℃. The optimal concentrations of various methanol dehydrogenase inhibitors such as potassium phosphate and EDTA were determined to be 100 and 0.5 mM, respectively, for an efficient production of methanol. Sodium formate (40 mM) as a reducing power source was added to enhance the conversion efficiency. A productivity of 49.0 mg/l·h, titer of 0.393 g methanol/l, and conversion of 73.8% (mol methanol/mol methane) were obtained under the optimized batch condition.
Keywords
Methane; methanol; methanol dehydrogenase; Methylosinus trichosporium OB3b; reducing power;
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Times Cited By KSCI : 2  (Citation Analysis)
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