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http://dx.doi.org/10.4014/jmb.1409.09012

Inhibitory Effect of Hexane Fraction from Myagropsis myagroides on Pancreatic α-Amylase In Vitro  

Pak, Won-Min (Department of Food Science and Technology/Institute of Food Science, Pukyong National University)
Kim, Koth-Bong-Woo Ri (Institute of Fisheries Sciences, Pukyong National University)
Kim, Min-ji (Institute of Fisheries Sciences, Pukyong National University)
Cho, Ji-Young (Department of Marine Biotechnology, Soonchunhyang University)
Ahn, Dong-Hyun (Department of Food Science and Technology/Institute of Food Science, Pukyong National University)
Publication Information
Journal of Microbiology and Biotechnology / v.25, no.3, 2015 , pp. 328-333 More about this Journal
Abstract
A Myagropsis myagroides (Mm) methanol extract showed α-amylase inhibitory activity of 13% at a concentration of 5 mg/ml. Results showed that the hexane fraction from the Mm methanol extract exhibited α-amylase inhibitory activity with an IC50 value of 4.24 mg/ml. The hexane fraction was separated using silica-gel column chromatography, and six subfractions were obtained. The fraction eluted with CHCl3:MeOH = 50:1 showed the highest α-amylase inhibitory activity with an IC50 value of 0.72 mg/ml. This fraction was purified using Sephadex LH-20 column chromatography and an octadecyl silica (ODS) Sepak cartridge, obtaining seven subfractions. Fraction (Fr.) 4 also showed a strong α-amylase inhibitory activity with an IC50 value of 0.75 mg/ml. Fr. 4 was purified by Sephadex LH-20 column chromatography and ODS Sepak cartridge, obtaining six subfractions. Fr. 4-2 was identified as sargachromanol I with an IC50 value of 0.40 mg/ml, and the inhibition pattern analyzed from Lineweaver-Burk plots revealed it to be an uncompetitive inhibitor. These results suggest that Mm has potential as a natural antidiabetes agent.
Keywords
α-Amylase; Myagropsis myagroides; sargachromanol I;
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