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http://dx.doi.org/10.4014/jmb.1505.05102

The Effects of Physicochemical Factors and Cell Density on Nitrite Transformation in a Lipid-Rich Chlorella  

Liang, Fang (Institute of Bioengineering, Zhengzhou Normal University)
Du, Kui (Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences)
Wen, Xiaobin (Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences)
Luo, Liming (Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences)
Geng, Yahong (Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences)
Li, Yeguang (Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences)
Publication Information
Journal of Microbiology and Biotechnology / v.25, no.12, 2015 , pp. 2116-2124 More about this Journal
Abstract
To understand the effects of physicochemical factors on nitrite transformation by microalgae, a lipid-rich Chlorella with high nitrite tolerance was cultured with 8 mmol/l sodium nitrite as sole nitrogen source under different conditions. The results showed that nitrite transformation was mainly dependent on the metabolic activities of algal cells rather than oxidation of nitrite by dissolved oxygen. Light intensity, temperature, pH, NaHCO3 concentrations, and initial cell densities had significant effects on the rate of nitrite transformation. Single-factor experiments revealed that the optimum conditions for nitrite transformation were light intensity: 300 μmol/m2/s; temperature: 30℃ pH: 7-8; NaHCO3 concentration: 2.0 g/l; and initial cell density: 0.15 g/l; and the highest nitrite transformation rate of 1.36 mmol/l/d was achieved. There was a positive correlation between nitrite transformation rate and the growth of Chlorella. The relationship between nitrite transformation rate (mg/l/d) and biomass productivity (g/l/d) could be described by the regression equation y = 61.3x (R2 = 0.9665), meaning that 61.3 mg N element was assimilated by 1.0 g dry biomass on average, which indicated that the nitrite transformation is a process of consuming nitrite as nitrogen source by Chlorella. The results demonstrated that the Chlorella suspension was able to assimilate nitrite efficiently, which implied the feasibility of using flue gas for mass production of Chlorella without preliminary removal of NOX.
Keywords
Chlorella; $NO_x$; nitrite transformation; flue gas;
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1 Chisti Y. 2007. Biodiesel from microalgae. Biotechnol. Adv. 25: 294-306.   DOI
2 Chen WM, Liu H, Zhang QM, Dai SG. 2011. Effect of nitrite on growth and microcystins production of Microcystis aeruginosa PCC7806. J. Appl. Phycol. 23: 665-671.   DOI
3 Brown LM. 1996. Uptake of carbon dioxide from flue gas by microalgae. Energy Conv. Manag. 37: 1363-1367.   DOI
4 Bernard O, Remond B. 2012. Validation of a simple model accounting for light and temperature effect on microalgal growth. Bioresour. Technol. 123: 520-527.   DOI
5 Lee JS, Kim DK, Lee JP, Park SC, Koh JH, Cho HS, Kim SW. 2002. Effects of SO2 and NO on growth of Chlorella sp. KR-1. Bioresour. Technol. 82: 1-4.   DOI
6 Lee JN, Lee JS, Shin CS, Park SC, Kim SW. 2000. Methods to enhance tolerances of Chlorella KR-1 to toxic compounds in flue gas. Appl. Biochem. Biotechnol. 10: 338-343.
7 Kurvits T, Marta T. 1998. Agricultural NH3 and NOx emissions in Canada. Environ. Pollut. 102: 187-194.   DOI
8 Kumar A, Ergas S, Yuan X, Sahu A, Zhang Q, Dewulf J, et al. 2010. Enhanced CO2 fixation and biofuel production via microalgae: recent developments and future directions. Trends Biotechnol. 28: 371-380.   DOI
9 Khalil ZI, Asker MMS, El-Sayed S, Kobbia IA. 2010. Effect of pH on growth and biochemical responses of Dunaliella bardawil and Chlorella ellipsoidea. World J. Microbiol. Biotechnol. 26: 1225-1231.   DOI
10 Kessler E, Czygan FC. 1968. The effect of iron supply on the activity of nitrate and nitrite reduction in green algae. Arch. Microbiol. 60: 282-284.
11 Jin HF, Santiago DEO, Park J, Lee K. 2008. Enhancement of nitric oxide solubility using Fe(II)-EDTA and its removal by green algae Scenedesmus sp. Biotechnol. Bioprocess Eng. 13: 48-52.   DOI
12 Han FF, Wang WL, Li YG, Shen GM, Wan MX, Wang J. 2013. Changes of biomass, lipid content and fatty acids composition under a light-dark cyclic culture of Chlorella pyrenoidosa in response to different temperature. Bioresour. Technol. 132: 182-189.   DOI
13 Dora J, Gostomczyk MA, Jakubiak M, Kordylewski W, Mista W, Tkaczuk M. 2009. Parametric studies of the effectiveness of NO oxidation process by ozone. Chem. Process Eng. 30: 621-633.
14 Olaizola M. 2003. Microalgal removal of CO2 from flue gases: changes in medium pH and flue gas composition do not appear to affect the photochemical yield of microalgal cultures. Biotechnol. Bioprocess Eng. 8: 360-367.   DOI
15 Ohkawa T, Hiramoto K, Kikugawa K. 2001. Standardization of nitric oxide aqueous solutions by modified Saltzman method. Nitric Oxide Biol. Chem. 5: 515-524.   DOI
16 Naguib YMA. 2000. Antioxidant activities of astaxanthin and related carotenoids. J. Agric. Food Chem. 48: 1150-1154.   DOI
17 Nagase H, Yoshihara K, Eguchi K, Yokota Y, Matsui R, Hirata K, Miyamoto K. 1997. Characteristics of biological NOx removal from flue gas in a Dunaliella tertiolecta culture system. J. Ferment. Bioeng. 83: 461-465.   DOI
18 Nagase H, Yoshihara K, Eguchi K, Okamoto Y, Murasaki S, Yamashita R, et al. 2001. Uptake pathway and continuous removal of nitric oxide from flue gas using microalgae. Biochem. Eng. J. 7: 241-246.   DOI
19 Matsumoto H, Hamasaki A, Sioji N, Ikuta Y. 1997. Influence of CO2, SO2 and NO in flue gas on microalgae productivity. J. Chem. Eng. Jpn. 30: 620-624.   DOI
20 Nagase H, Eguchi K, Yoshihara K, Hirata K, Miyamoto K. 1998. Improvement of microalgal NOx removal in bubble column and airlift reactors. J. Ferment. Bioeng. 86: 421-423.   DOI
21 Mallick N, Rai LC, Mohn FH, Soeder CJ. 1999. Studies on nitric oxide (NO) formation by the green alga Scenedesmus obliquus and the diazotrophic cyanobacterium Anabaena doliolum. Chemosphere 39: 1601-1610.   DOI
22 Liang F, Wen X, Luo L, Geng Y, Li Y. 2014. Physicochemical effects on sulfite transformation in a lipid-rich Chlorella sp. strain. Chin. J. Oceanol. Limnol. 32: 1288-1296.   DOI
23 Ward BB. 2008. Nitrification in marine systems, pp. 199-261. In Capone GD, Bronk AD, Mulholland RM, Carpenter JE (eds.). Nitrogen in the Marine Environment, 2nd Ed. Academic Press, Salt Lake City, UT.
24 Wang B, Li Y, Wu N, Lan CQ. 2008. CO2 bio-mitigation using microalgae. Appl. Microbiol. Biotechnol. 79: 707-718.   DOI
25 Van Den Hende S, Vervaeren H, Boon N. 2012. Flue gas compounds and microalgae: (bio-)chemical interactions leading to biotechnological opportunities. Biotechnol. Adv. 30: 1405-1424.   DOI
26 Tang D, Han W, Li P, Miao X, Zhong J. 2011. CO2 biofixation and fatty acid composition of Scenedesmus obliquus and Chlorella pyrenoidosa in response to different CO2 levels. Bioresour. Technol. 102: 3071-3076.   DOI
27 PRC National Standard. 1987. Water quality - determination of nitrate - spectrophotometric method with phenol disulfonic acid. Mnistry of Environmental Protection, China.
28 Sakihama Y, Nakamura S, Yamasaki H. 2002. Nitric oxide production mediated by nitrate reductase in the green alga Chlamydomonas reinhardtii: an alternative NO production pathway in photosynthetic organisms. Plant Cell Physiol. 43: 290-297.   DOI
29 Radmann EM, Costa JAV. 2008. Lipid content and fatty acids composition variation of microalgae exposed to CO2, SO2 and NO. Quim. Nova 31: 1609-1612.   DOI
30 Rabinowitch HD, Fridovich I. 1985. Growth of Chlorella sorokiniana in the presence of sulfite elevates cell content of superoxide dismutase and imparts resistance towards paraquat. Planta 164: 524-528.   DOI
31 PRC National Standard. 1987. Water quality - determination of nitrogen (nitrite) - spectrophotometric method. Mnistry of Environmental Protection, China.
32 Zhu XY, Zhang D, Liang F, Wen XB, Li YG, Geng YH. 2014. Effects of environmental factors on the photosynthesis of Chlorella sp. XQ-20044. Plant Sci. J. 32: 74-79. (In Chinese with English abstract.)
33 Zhang BY, Geng YH, Li ZK, Hu HJ, Li YG. 2009. Production of astaxanthin from Haematococcus in open pond by two-stage growth one-step process. Aquaculture 295: 275-281.   DOI
34 Yoshihara KI, Nagase H, Eguchi K, Hirata K, Miyamoto K. 1996. Biological elimination of nitric oxide and carbon dioxide from flue gas by marine microalga NOA-113 cultivated in a long tubular photobioreactor. J. Ferment. Bioeng. 82: 351-354.   DOI
35 Yang SL, Wang J, Cong W, Cai ZL, Fan OY. 2004. Utilization of nitrite as a nitrogen source by Botryococcus braunii. Biotechnol. Lett. 26: 239-243.   DOI
36 Wen X, Geng Y, Li Y. 2014. Enhanced lipid production in Chlorella pyrenoidosa by continuous culture. Bioresour. Technol. 161: 297-303.   DOI
37 Wodzinski RS, Labeda DP, Alexander M. 1978. Effects of low concentrations of bisulfite-sulfite and nitrite on microorganisms. Appl. Environ. Microbiol. 35: 718-723.