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http://dx.doi.org/10.4014/jmb.1505.05069

Melanin Biosynthesis Inhibition Effects of Ginsenoside Rb2 Isolated from Panax ginseng Berry  

Lee, Dae Young (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Jeong, Yong Tae (HK BIO, Business Incubator, Daegu Haany University)
Jeong, Sang Chul (Freshwater Bioresources Utilization Division, Nakdonggang National Institute of Biological Resources)
Lee, Mi Kyoung (Cancer Preventive Material Development Research Center, College of Oriental Medicine, Kyung Hee University)
Min, Jin Woo (Research & Development Center, GFC Co., Ltd.)
Lee, Jae Won (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Kim, Geum Soog (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Lee, Seung Eun (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Ahn, Young Sup (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Kang, Hee Cheol (Research & Development Center, GFC Co., Ltd.)
Kim, Jin Hee (College of Herbal Bio-industry, Daegu Haany University)
Publication Information
Journal of Microbiology and Biotechnology / v.25, no.12, 2015 , pp. 2011-2015 More about this Journal
Abstract
Ginsenoside Rb2 (Gin-Rb2) was purified from the fruit extract of Panax ginseng. Its chemical structure was measured by spectroscopic analysis, including HR-FAB-MS, 1H-NMR, and IR spectroscopy. Gin-Rb2 decreased potent melanogenesis in melan-a cells, with 23.4% at 80 μM without cytotoxicity. Gin-Rb2 also decreased tyrosinase and MITF protein expression in melan-a cells. Furthermore, Gin-Rb2 presented inhibition of the body pigmentation in the zebrafish in vivo system and reduced melanin contents and tyrosinase activity. These results show that Gin-Rb2 isolated from P. ginseng may be an effective skin-whitening agent via the in vitro and in vivo systems.
Keywords
Ginsenoside Rb2; Panax ginseng; melanin biosynthesis; melan-a cell; zebrafish;
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