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http://dx.doi.org/10.4014/jmb.1504.04047

Inhibitory Effect of Lactobacillus plantarum Extracts on HT-29 Colon Cancer Cell Apoptosis Induced by Staphylococcus aureus and Its Alpha-Toxin  

Kim, Hangeun (Skin Biotechnology Center, Kyung Hee University)
Kim, Hye Sun (Department of Neurosurgery, Ajou University School of Medicine)
Park, Woo Jung (Department of Marine Food Science and Technology, Gangneung-Wonju National University)
Chung, Dae Kyun (Skin Biotechnology Center, Kyung Hee University)
Publication Information
Journal of Microbiology and Biotechnology / v.25, no.11, 2015 , pp. 1849-1855 More about this Journal
Abstract
Staphylococcus aureus plays an important role in sepsis, septic shock, pneumonia, and wound infections. Here, we demonstrate that Lactobacillus plantarum extracts inhibited S. aureus-induced cell death of a human epithelial cell line, HT-29. In particular, we have shown that S. aureus-induced cell death was abolished by neutralization of α-toxin, indicating that α-toxin is the major mediator of S. aureus-induced cell death. DNA fragmentation experiment and caspase assay revealed that the S. aureus-induced cell death was apoptosis. L. plantarum extracts inhibited the generation of effector caspase-3 and the initiator caspase-9 in S. aureus- or α-toxin-induced cell death. Moreover, expression of Bcl-2, an anti-apoptotic protein, was activated in L. plantarum extract-treated cells as compared with the S. aureus- or α-toxin-treated only cells. Furthermore, S. aureus-induced apoptosis was efficiently inhibited by lipoteichoic acid and peptidoglycan of L. plantarum. Together, our results suggest that L. plantarum extracts can inhibit the S. aureus-mediated apoptosis, which is associated with S. aureus spreading, in intestinal epithelial cells, and may provide a new therapeutic reagent to treat bacterial infections.
Keywords
Staphylococcus aureus; α-toxin; Lactobacillus plantarum; crude extract; apoptosis; colon cancer cells;
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