[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.4014/jmb.1503.03033

Anticancer and Anti-Inflammatory Activity of Probiotic Lactococcus lactis NK34

Han, Kyoung Jun (Department of Food Science and Biotechnology of Animal Resources, Konkuk University)
Lee, Na-Kyoung (Department of Food Science and Biotechnology of Animal Resources, Konkuk University)
Park, Hoon (Department of Food Science, Sun Moon University)
Paik, Hyun-Dong (Department of Food Science and Biotechnology of Animal Resources, Konkuk University)

Publication Information

Journal of Microbiology and Biotechnology / v.25, no.10, 2015 , pp. 1697-1701 More about this Journal

Abstract

The anticancer and anti-inflammatory activities of probiotic Lactococcus lactis NK34 were demonstrated. Treatment of cancer cells such as SK-MES-1, DLD-1, HT-29, LoVo, AGS, and MCF-7 cells with 10⁶ CFU/well of L. lactis NK34 resulted in strong inhibition of proliferation (>77% cytotoxicity, p < 0.05). The anti-inflammatory activity of L. lactis NK34 was also demonstrated in lipopolysaccharide-induced RAW 264.7 cells, where the production of nitric oxide and proinflammatory cytokines (tumor necrosis factor-α, interleukin-18, and cyclooxygenase-2) was reduced. These results suggest that L. lactis NK34 could be used as a probiotic microorganism to inhibit the proliferation of cancer cells and production of proinflammatory cytokines.

Keywords

Probiotic; Lactococcus lactis; anticancer activity; anti-inflammatory activity; cytokine;

Citations & Related Records

Reference

1	Chang JH, Shin YY, Chae SK, Chee KM. 2010. Probiotic characteristics of lactic acid isolated from kimchi. J. Appl. Microbiol. 109: 220-230. DOI
2	Lee NK, Noh JE, Choi GH, Park E, Chang HI, Yun CW, et al. 2007. Potential probiotic properties of Lactococcus lactis NK34 isolated from jeotgal. Food Sci. Biotechnol. 16: 843-847.
3	Lee NK, Kim SY, Han KJ, Eom SJ, Paik HD. 2014. Probiotic potential of Lactobacillus strains with anti-allergic effects from kimchi for yogurt starters. LWT Food Sci. Technol. 58: 130-134. DOI
4	Zhou X, Wang Y, Li W. 2010. Inhibition ability of probiotic, Lactococcus lactis, against A. hydrophila and study of its immunostimulatory effect in tilapia (Oreochromis niloticus). Int. J. Eng. Sci. Technol. 2: 73-80.
5	Baumgart DC, Thomas S, Przesdzing I, Metzke D, Bielecki C, Lehmann SM, et al. 2009. Exaggerated inflammatory response of primary human myeloid dendritic cells to lipopolysaccharide in patients with inflammatory bowel disease. Clin. Exp. Immunol. 157: 423-436. DOI
6	Commane D, Hughes R, Shortt C, Rowland I. 2005. The potential mechanisms involved in the anti-carcinogenic action of probiotics. Mut. Res. 591: 276-289. DOI
7	Festen EAM, Szperl AM, Weersma RK, Wikmenga C, Wapenaar MC. 2009. Inflammatory bowel disease and celiac disease: overlaps in the pathology and genetics, and their potential drug targets. Curr. Drug Targets Immune Endocr. Metab. Disord. 9: 199-218. DOI
8	Heo WS, Kim YR, Kim EY, Bai S, Kong IS. 2013. Effects of dietary probiotic, Lactococcus lactis subsp. lactis I2, supplementation on the growth and immune response of olive flounder (Paralichthys olivaceus). Aquaculture 376-379: 20-24. DOI
9	Lakatos L, Lakatos PL. 2006. Is the incidence and prevalence of inflammatory bowel diseases increasing in Eastern Europe? Postgrad. Med. J. 82: 332-337. DOI
10	Otte JM, Mahjurian-Namari R, Brand S, Werner I, Schmidt WE, Schmitz F. 2009. Probiotics regulate the expression of COX-2 in intestinal epithelial cells. Nutr. Cancer 61: 103-113. DOI
11	Ouwehand AC. 2011. Allergenic effects of probiotics. J. Nutr. 137: 794S-797S.
12	Lee NK, Son SH, Jeon EB, Kim GH, Lee JY, Paik HD. 2015. The prophylactic effect of probiotic Bacillus polyfermenticus KU3 against cancer cells. J. Funct. Foods 14: 513-518. DOI
13	Marcinkiewicz J, Ciszek M, Bobek M, Strus M, Heczko PB, Kurnyta M, et al. 2007. Differential inflammatory mediator response in vitro from murine macrophages to lactobacilli and pathogenic intestinal bacteria. Int. J. Exp. Pathol. 88: 155-164. DOI
14	O’Sullivan GC, Kelly P, O’Halloran S, Collins C, Collins JK, Dunne C, Shanahan F. 2005. Probiotics: an emerging therapy. Curr. Pharm. Des. 11: 3-10. DOI
15	Shukla R, Iliev I, Goyal A. 2014. Leuconostoc mesenteroides NRRL B-1149 as probiotic and its dextran with anticancer properties. J. Biosci. Biotechnol. 3: 79-87.
16	Westbrook AM, Szakmary A, Schiestl RH. 2010. Mechanisms of intestinal inflammation and development of associated cancers: lessons learned from mouse models. Mutat. Res. 705: 40-59. DOI

Reference

1	(2015) Current opinion in oncology Probiotics-mediated suppression of cancer / 29 (1) , 62
1	(2015) Allergy A prospective microbiome‐wide association study of food sensitization and food allergy in early childhood / 73 (1) , 145
None	(2015) Frontiers in pharmacology A Controlled Fermented <I>Samjunghwan</I> Herbal Formula Ameliorates Non-alcoholic Hepatosteatosis in HepG2 Cells and OLETF Rats / 9 (None) , 596
3	(2015) Beneficial microbes Appropriate dose of <I>Lactobacillus buchneri</I> supplement improves intestinal microbiota and prevents diarrhoea in weaning Rex rabbits / 9 (3) , 401
19	(2015) Ecology and evolution Fecal microbiota associated with phytohaemagglutinin‐induced immune response in nestlings of a passerine bird / 8 (19) , 9793
None	(2015) Frontiers in microbiology Mucosa-Associated Microbiota in Gastric Cancer Tissues Compared With Non-cancer Tissues / 10 (None) , 1261
2	(2015) Oncology reviews Potential effect of probiotics in the treatment of breast cancer / 13 (2) , None
8	(2019) Current microbiology Probiotic Bacteria: A Promising Tool in Cancer Prevention and Therapy / 76 (8) , 939
None	(2020) Food & nutrition research Bile salt hydrolase-overexpressing <I>Lactobacillus</I> strains can improve hepatic lipid accumulation <I>in vitro</I> in an NAFLD cell model / 64 (None) , 10.29219/fnr.v64.3751
3	(2015) Nature microbiology Endogenous murine microbiota member <I>Faecalibaculum rodentium</I> and its human homolog protect from intestinal tumor growth / 5 (3) , 511
4	(2015) Journal of microbiology and biotechnology Antioxidant and Anti-Inflammatory Effect of Probiotic Lactobacillus plantarum KU15149 Derived from Korean Homemade Diced-Radish Kimchi / 30 (4) , 591
18	(2015) Applied microbiology and biotechnology Unraveling microbial fermentation features in kimchi: from classical to meta-omics approaches / 104 (18) , 7731
1	(2015) Cancers The Role of Probiotics in Cancer Prevention / 13 (1) , 20
None	(2015) Frontiers in microbiology Role of the Gastric Microbiome in Gastric Cancer: From Carcinogenesis to Treatment / 12 (None) , 641322
3	(2015) Probiotics and antimicrobial proteins Neuroprotective Effects of Heat-Killed Lactobacillus plantarum 200655 Isolated from Kimchi Against Oxidative Stress / 13 (3) , 788
12	(2015) Cancers The Anti-Tumor Effect of Lactococcus lactis Bacteria-Secreting Human Soluble TRAIL Can Be Enhanced by Metformin Both In Vitro and In Vivo in a Mouse Model of Human Colorectal Cancer / 13 (12) , 3004
11	(2015) Critical reviews in food science and nutrition Molecular mechanisms of postbiotics in colorectal cancer prevention and treatment / 61 (11) , 1787
7	(2021) Gut: journal of the British Society of Gastroenterology Long-term dietary patterns are associated with pro-inflammatory and anti-inflammatory features of the gut microbiome / 70 (7) , 1287
13	(2015) Cancers Probiotics: A Promising Candidate for Management of Colorectal Cancer / 13 (13) , 3178
31	(2015) Environmental science and pollution research international Kefir milk alleviates benzene-induced immunotoxicity and hematotoxicity in rats / 28 (31) , 42230
10	(2015) Antibiotics Enterococcus spp. as a Producer and Target of Bacteriocins: A Double-Edged Sword in the Antimicrobial Resistance Crisis Context / 10 (10) , 1215
1	(2015) BMC microbiology Oral microbiota and Helicobacter pylori in gastric carcinogenesis: what do we know and where next? / 21 (1) , 71