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http://dx.doi.org/10.4014/jmb.1504.04007

Identification of Actinobacillus pleuropneumoniae Genes Preferentially Expressed During Infection Using In Vivo-Induced Antigen Technology (IVIAT)  

Zhang, Fei (Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricutural University)
Zhang, Yangyi (Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricutural University)
Wen, Xintian (Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricutural University)
Huang, Xiaobo (Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricutural University)
Wen, Yiping (Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricutural University)
Wu, Rui (Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricutural University)
Yan, Qigui (Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricutural University)
Huang, Yong (Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricutural University)
Ma, Xiaoping (Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricutural University)
Zhao, Qin (Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricutural University)
Cao, Sanjie (Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricutural University)
Publication Information
Journal of Microbiology and Biotechnology / v.25, no.10, 2015 , pp. 1606-1613 More about this Journal
Abstract
Porcine pleuropneumonia is an infectious disease caused by Actinobacillus pleuropneumoniae. The identification of A. pleuropneumoniae genes, specially expressed in vivo, is a useful tool to reveal the mechanism of infection. IVIAT was used in this work to identify antigens expressed in vivo during A. pleuropneumoniae infection, using sera from individuals with chronic porcine pleuropneumonia. Sequencing of DNA inserts from positive clones showed 11 open reading frames with high homology to A. pleuropneumoniae genes. Based on sequence analysis, proteins encoded by these genes were involved in metabolism, replication, transcription regulation, and signal transduction. Moreover, three function-unknown proteins were also indentified in this work. Expression analysis using quantitative real-time PCR showed that most of the genes tested were up-regulated in vivo relative to their expression levels in vitro. IVI (in vivo-induced) genes that were amplified by PCR in different A. pleuropneumoniae strains showed that these genes could be detected in almost all of the strains. It is demonstrated that the identified IVI antigen may have important roles in the infection of A. pleuropneumoniae.
Keywords
Actinobacillus pleuropneumoniae; in vivo-induced genes; differential expression;
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