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http://dx.doi.org/10.4014/jmb.1404.04004

Enzymatic Synthesis of 2-Phenoxyethanol Galactoside by Whole Cells of ${\beta}$-Galactosidase-Containing Escherichia coli  

Lee, Hyang-Yeol (Department of Biotechnology, Korea National University of Transportation)
Jung, Kyung-Hwan (Department of Biotechnology, Korea National University of Transportation)
Publication Information
Journal of Microbiology and Biotechnology / v.24, no.9, 2014 , pp. 1254-1259 More about this Journal
Abstract
We investigated whether ${\beta}$-galactosidase (${\beta}$-gal)-containing Escherichia coli cells could transfer a galactose to 2-phenoxyethanol, resulting in 2-phenoxyethanol galactoside (PE-Gal). PE-Gal was confirmed by liquid chromatography-mass spectrometry. In addition, we also confirmed that a galactose molecule was covalently bonded with PE during thin-layer chromatography analysis of the ${\beta}$-gal hydrolysate of PE-Gal. The yield for PE-Gal synthesis was about 37.5% (weight basis), which was about 7-8 times greater than that of a previous report. In addition, the concentration of ${\beta}$-gal (0.96 U/ml) used in this PE-Gal synthesis was about 20 times less than that in a previous report.
Keywords
2-Phenoxyethanol; ${\beta}$-galactosidase; transgalactosylation; 2-phenoxyethanol galactoside; Escherichia coli;
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Times Cited By KSCI : 5  (Citation Analysis)
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