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http://dx.doi.org/10.4014/jmb.1402.02007

Effects of Temperature and Supplementation with Skim Milk Powder on Microbial and Proteolytic Properties During Storage of Cottage Cheese  

Oh, Nam Su (R&D Center, Seoul Dairy Cooperative)
Lee, Hyun Ah (R&D Center, Seoul Dairy Cooperative)
Myung, Jae Hee (R&D Center, Seoul Dairy Cooperative)
Joung, Jae Yeon (R&D Center, Seoul Dairy Cooperative)
Lee, Ji Young (R&D Center, Seoul Dairy Cooperative)
Shin, Yong Kook (R&D Center, Seoul Dairy Cooperative)
Baick, Seung Chun (R&D Center, Seoul Dairy Cooperative)
Publication Information
Journal of Microbiology and Biotechnology / v.24, no.6, 2014 , pp. 795-802 More about this Journal
Abstract
The aim of this study was to determine the effects of temperature and supplementation with skim milk powder (SMP) on the microbial and proteolytic properties during the storage of cottage cheese. Cottage cheese was manufactured using skim milk with 2% SMP and without SMP as the control, and then stored at $5^{\circ}C$ or $12^{\circ}C$ during 28 days. The chemical composition of the cottage cheese and the survival of the cheese microbiota containing starter lactic acid bacteria (SLAB) and non-starter culture lactic acid bacteria (NSLAB) were evaluated. In addition, changes in the concentration of lactose and lactic acid were analyzed, and proteolysis was evaluated through the measurement of acid soluble nitrogen (ASN) and non-protein nitrogen (NPN), as well as electrophoresis profile analysis. The counts of SLAB and NSLAB increased through the addition of SMP and with a higher storage temperature ($12^{\circ}C$), which coincided with the results of the lactose decrease and lactic acid production. Collaborating with these microbial changes, of the end of storage for 28 days, the level of ASN in samples at $12^{\circ}C$ was higher than those at $5^{\circ}C$. The NPN content was also progressively increased in all samples stored at $12^{\circ}C$. Taken together, the rate of SLAB and NSLAB proliferation during storage at $12^{\circ}C$ was higher than at $5^{\circ}C$, and consequently it led to increased proteolysis in the cottage cheese during storage. However, it was relatively less affected by SMP fortification. These findings indicated that the storage temperature is the important factor for the quality of commercial cottage cheese.
Keywords
Cottage cheese; storage temperature; non-starter lactic acid bacteria; proteolysis;
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