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http://dx.doi.org/10.4014/jmb.1311.11107

Novel Phage Display-Derived H5N1-Specific scFvs with Potential Use in Rapid Avian Flu Diagnosis  

Wu, Jie (Center for Disease Control and Prevention)
Zeng, Xian-Qiao (Center for Disease Control and Prevention)
Zhang, Hong-Bin (Gangzhou General Hospital of Guangzhou Military District)
Ni, Han-Zhong (Center for Disease Control and Prevention)
Pei, Lei (International Dialogues and Conflict Management)
Zou, Li-Rong (Center for Disease Control and Prevention)
Liang, Li-Jun (Center for Disease Control and Prevention)
Zhang, Xin (Center for Disease Control and Prevention)
Lin, Jin-Yan (Center for Disease Control and Prevention)
Ke, Chang-Wen (Center for Disease Control and Prevention)
Publication Information
Journal of Microbiology and Biotechnology / v.24, no.5, 2014 , pp. 704-713 More about this Journal
Abstract
The highly pathogenic avian influenza A (HPAI) viruses of the H5N1 subtype infect poultry and have also been spreading to humans. Although new antiviral drugs and vaccinations can be effective, rapid detection would be more efficient to control the outbreak of infections. In this study, a phage-display library was applied to select antibody fragments for HPAI strain A/Hubei/1/2010. As a result, three clones were selected and sequenced. A hemagglutinin inhibition assay of the three scFvs revealed that none exhibited hemagglutination inhibition activity towards the H5N1 virus, yet they showed a higher binding affinity for several HPAI H5N1 strains compared with other influenza viruses. An ELISA confirmed that the HA protein was the target of the scFvs, and the results of a protein structure simulation showed that all the selected scFvs bound to the HA2 subunit of the HA protein. In conclusion, the three selected scFVs could be useful for developing a specific detection tool for the surveillance of HPAI epidemic strains.
Keywords
H5N1 virus; antibody; phage library; diagnosis;
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1 Liu H, Zheng X, Zhang F, Yu L, Zhang X, Dai H, et al. 2013. Selection and characterization of single-chain recombinant antibodies against spring viraemia of carp virus from mouse phage display library. J. Virol. Methods 194: 178-184.   DOI
2 Luka J, Arlen PM, Bristol A. 2011. Development of a serum biomarker assay that differentiates tumor-associated MUC5AC (NPC-1C ANTIGEN) from normal MUC5AC. J. Biomed. Biotechnol. 2011: 934757.
3 Okuno Y, Isegawa Y, Sasao F, Ueda S. 1993. A common neutralizing epitope conserved between the hemagglutinins of influenza A virus H1 and H2 strains. J. Virol. 67: 2552-2558.
4 Pettersen E F, G oddard T D, H uang CC , C uoch G S, Greenblatt DM, Meng EC, Ferrin TE. 2004. UCSF Chimera - a visualization system for exploratory research and analysis. J. Comput. Chem. 25: 1605-1612.   DOI   ScienceOn
5 Raghava GP, Agrewala JN. 1994. Method for determining the affinity of monoclonal antibody using non-competitive ELISA: a computer program. J. Immunoassay 15: 115-128.   DOI
6 Russ G, Polakova K, Kostolansky F, Styk B, Vancikova M. 1987. Monoclonal antibodies to glycopolypeptides HA1 and HA2 of influenza virus haemagglutinin. Acta Virol. 31: 374-386.
7 Simmons PC, Bernasconi LN, Suguitan Jr LA, Mills K, Ward MJ, Chau VN. 2007. Prophylactic and therapeutic efficacy of human monoclonal antibodies against H5N1 influenza. PLoS Med. 4: e178.   DOI
8 Throsby M, van den Brink E, Jongeneelen M, Poon LLM, Alard P. 2008. Heterosubtypic neutralizing monoclonal antibodies cross-protective against H5N1 and H1N1 recovered from human IgM memory B cells. PLoS One 3: e3942.   DOI
9 Wang W, Ren P, Mardi S, Hou L, Tsai C, Chan KH, et al. 2009. Design of multiplexed detection assays for identification of avian influenza A virus subtypes pathogenic to humans by SmartCycler real-time reverse transcription-PCR. J. Clin. Microbiol. 47: 86-92.   DOI
10 Ekiert DC, Friesen RH, Bhabha G, Kwaks T, Jongeneelen M, Yu W, et al. 2011. A highly conserved neutralizing epitope on group 2 influenza A viruses. Science 333: 843-850.   DOI
11 Fouchier R, Kuiken T, Rimmelzwaan G, Osterhaus A. 2005. Global task force for influenza. Nature 435: 419-420.   DOI
12 Gavin PJ, Thomson Jr R. B.2003. Review of rapid diagnostic tests for influenza. Clin. Appl. Immunol. Rev. 4: 151-172.
13 Herfst S, Schrauwen EJ, Linster M, Chutinimitkul S, de Wit E, Munster VJ, et al. 2012. Airborne transmission of influenza A/H5N1 virus between ferrets. Science 336: 1534-1541.   DOI
14 Hu H, Voss J, Zhang G, Buchy P, Zuo T, Wang L, et al. 2012. A human antibody recognizing a conserved epitope of H5 hemagglutinin broadly neutralizes highly pathogenic avian influenza H5N1 viruses. J. Virol. 86: 2978-2989.   DOI
15 Jin ML, Chen HC, Xiong YZ. 2006. The study of pathogenic features of avian influenza virus subtype H5N1, gene cloning, sequence analysis of HA and NA, and the construction of recombinant vaccine strain. Doctoral Thesis. Animal Genetics and Breeding, Huazhong Agricultural University, China.
16 Ahmad ZA, Yeap SK, Ali AM, Ho WY, Alitheen NB, Hamid M. 2012. scFv antibody: principles and clinical application. Clin. Dev. Immunol. 2012: 1-15.
17 Kaku Y, Noguchi A, Okutani A, Inoue S, Tanabayashi K, Yamamoto Y, et al. 2012. Altered specificity of single-chain antibody fragments bound to pandemic H1N1-2009 influenza virus after conversion of the phage bound to the soluble form. BMC Res. Notes 4: 483.
18 Lim AP, Chan CE, Wong SK, Chan AH, Ooi EE, Hanson BJ. 2008. Neutralizing human monoclonal antibody against H5N1 influenza HA selected from a Fab-phage display library. J. Virol. 5: 1-10.   DOI
19 Liu FF, Jin ML, Zhang AD, Dan HB, Chen HC. 2006. Preparation and partial characterization of monoclonal antibodies specific for nuclear protein of avian influenza virus. Chin. J. Cell Mol. Immunol. 22: 648-653.
20 Beatty JD, Beatty BG, Vlahos WG. 1987. Measurement of monoclonal antibody affinity by non-competitive enzyme immunoassay. J. Immunol. Methods 100: 173-179.   DOI
21 Chen Y, Luo W, Song H, Yin B, Tang J , Chen Y, et al. 2011. Mimotope ELISA for detection of broad spectrum antibody against avian H5N1 influenza virus. Plos One 6: e24144.   DOI
22 Cho CH, Woo MK, Kim JY, Cheong S, Lee CK, An SA, et al. 2013. Evaluation of five rapid diagnostic kits for influenza A/B virus. J. Virol. Methods 187: 51-56.   DOI
23 Corti D, Voss J, Gamblin SJ, Codoni G, Macagno A, Jarrossay D, et al. 2011. A neutralizing antibody selected from plasma cells that binds to group 1 and group 2 influenza A hemagglutinins. Science 333: 850-856.   DOI
24 De Jong JC, Claas EC, Osterhaus AD, Webster RG, Lim WL. 1997. A pandemic warning? Nature 389: 554.
25 De Wildt RM, Mundy CR, Gorick BD, Tomlinson IM. 2000. Antibody arrays for high-throughput screening of antibodyantigen interactions. Nat. Biotechnol. 18: 989-994.   DOI   ScienceOn
26 DuBois RM, Zaraket H, Reddivari M, Heath RJ, White SW, Russell CJ. 2011. Acid stability of the hemagglutinin protein regulates H5N1 influenza virus pathogenicity. PLoS Pathog. e1002398.
27 Wu RW, Bi DR. 2006. Development of indirect enzymelinked immunosorbent assay with nucleoprotein as antigen for detection and quantifications of antibodies against avian influenza virus. Doctoral Thesis. Veterinary Medicine, Huazhong Agricultural University, China.
28 Webster RG, Govorkova EA. 2006. H5N1 influenza - continuing evolution and spread. N. Engl. J. Med. 355: 2174-2177.   DOI
29 World Health Organization. 2012. Cumulative number of confirmed human cases of avian influenza A (H5N1) reported to WHO, 2003-2012. Accessible at http:// www.who.int/influenza/human animal interface/EN GIP 20120116CumulativeNumberH5N1cases.pdf.
30 Wu D, L i G, Qin C , Ren X. 2 011. Phage displayed p eptides to avian H5N1 virus distinguished the virus from other viruses. PLoS One 6: e23058.   DOI
31 Xu R, Ekiert DC, Krause JC, Hai R, Crowe Jr JE, Wilson IA. 2010. Structural basis of pre-existing immunity to the 2009 H1N1 pandemic influenza virus. Science 328: 357-360.   DOI
32 Yodsheewan R, Maneewatch S, Srimanote P, Thueng-In K, Songserm T, Dong-Din-On F, et al. 2013. Human monoclonal ScFv specific to NS1 protein inhibits replication of influenza viruses across types and subtypes. Antiviral Res. 100: 226-237.   DOI
33 Yu ZJ, Jin ML, Xu XJ, Zhang RH, Chen HC. 2005. A latex agglutination test for detection of hemagglutinin serum antibodies to H5 Avian influenza virus in chicken. Acta Microbiol. Sin. 45: 942-946.
34 Zhang RH, Jin ML, Chen HC. 2005. Gene cloning, expression and subtype detection of hemmaglutinin of avian influenza virus. Master Thesis. Veterinary Medicine, Huazhong Agricultural University, China.
35 Zhang Y. 2008. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics 9: 40.   DOI   ScienceOn
36 Kirsch M, Zaman M, Meier D, Dübel S, Hust M. 2005. Parameters affecting the display of antibodies on phage. J. Immun. Methods 301: 173-185.   DOI