[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.4014/jmb.1401.01023

Characterization of CTX-M-Type Extended-Spectrum Beta-Lactamase-Producing Diarrheagenic Escherichia coli Isolates in the Republic of Korea During 2008-2011

Kim, Jin Seok (Division of Enteric Diseases, Center for Infectious Diseases, Korea National Institute of Health)
Kim, Junyoung (Division of Enteric Diseases, Center for Infectious Diseases, Korea National Institute of Health)
Kim, Soo-Jin (Division of Enteric Diseases, Center for Infectious Diseases, Korea National Institute of Health)
Jeon, Se-Eun (Division of Enteric Diseases, Center for Infectious Diseases, Korea National Institute of Health)
Oh, Kyung Hwan (Division of Enteric Diseases, Center for Infectious Diseases, Korea National Institute of Health)
Cho, Seung-Hak (Division of Enteric Diseases, Center for Infectious Diseases, Korea National Institute of Health)
Kang, Yeon-Ho (Division of Enteric Diseases, Center for Infectious Diseases, Korea National Institute of Health)
Han, Soon Young (Division of Enteric Diseases, Center for Infectious Diseases, Korea National Institute of Health)
Chung, Gyung Tae (Division of Enteric Diseases, Center for Infectious Diseases, Korea National Institute of Health)

Publication Information

Journal of Microbiology and Biotechnology / v.24, no.3, 2014 , pp. 421-426 More about this Journal

Abstract

To characterize the extended-spectrum beta-lactamases (ESBLs) in diarrheagenic Escherichia coli from Korea in 2008-2011, we screened seven enterotoxigenic E. coli (ETEC) and one enteroaggregative E. coli (EAEC) that produce ESBLs from a nationwide survey. All eight isolates produced CTX-M-type ESBLs, including CTX-M-12 (n = 4), CTX-M-14 (n = 2), and CTX-M-15 (n = 2). PCR-based replicon typing indicated that the $bla_{CTX-M-12}$ genes of four ETEC isolates were carried on a conjugative IncF plasmid, whereas the $bla_{CTX-M-14}$ of one EAEC was located on an IncK plasmid. This is the first report of the occurrence of $bla_{CTX-M}$ genes in clinical isolates of EAEC in Korea. The ESBL-producing isolates were shown to be different based on pulsed-field gel electrophoresis and multilocus sequence typing, whereas the four isolates with CTX-M-12 were clonally related. These observations raise an alarm for the spread of plasmid-mediated resistance to ESBL among diarrheagenic E. coli.

Keywords

ESBL; CTX-M; IncF; ETEC; EAEC;

Citations & Related Records

Times Cited By KSCI : 1 (Citation Analysis)

Reference
Cited By KSCI

1	Villegas MV, Correa A, Perez F, Zuluaga T, Radice M, Gutkind G, et al. 2004. CTX-M-12 beta-lactamase in a Klebsiella pneumoniae clinical isolate in Colombia. Antimicrob. Agents Chemother. 48: 629-631. DOI ScienceOn
2	Pai H , Choi E H, Lee HJ, Hong JY, J acoby GA. 2001. Identification of CTX-M-14 extended-spectrum beta-lactamase in clinical isolates of Shigella sonnei, Escherichia coli, and Klebsiella pneumoniae in Korea. J. Clin. Microbiol. 39: 3747-3749. DOI ScienceOn
3	Nataro JP, Steiner T, Guerrant RL. 1998. Enteroaggregative Escherichia coli. Emerg. Infect. Dis. 4: 251-261. DOI ScienceOn
4	Qadri F, Svennerholm AM, Faruque AS, Sack RB. 2005. Enterotoxigenic Escherichia coli in developing countries: epidemiology, microbiology, clinical features, treatment, and prevention. Clin. Microbiol. Rev. 18: 465-483. DOI ScienceOn
5	Schmidt H, Knop C, Franke S, Aleksic S, Heesemann J, Karch H. 1995. Development of PCR for screening of enteroaggregative Escherichia coli. J. Clin. Microbiol. 33: 701-705.
6	Shin J, Choi MJ, Ko KS. 2012. Replicon sequence typing of IncF plasmids and the genetic environments of blaCTX-M- 15 indicate multiple acquisitions of blaCTX-M-15 in Escherichia coli and Klebsiella pneumoniae isolates from South Korea. J. Antimicrob. Chemother. 67: 1853-1857. DOI ScienceOn
7	Orskov I, Orskov F, Jann B, Jann K. 1977. Serology, chemistry, and genetics of O and K antigens of Escherichia coli. Bacteriol. Rev. 41: 667-710.
8	Song W, Kim J, Bae IK, Jeong SH, Seo YH, Shin JH, et al. 2011. Chromosome-encoded AmpC and CTX-M extendedspectrum beta-lactamases in clinical isolates of Proteus mirabilis from Korea. Antimicrob. Agents Chemother. 55: 1414- 1419. DOI ScienceOn
9	Song W, Lee H, Lee K, Jeong SH, Bae IK, Kim JS, Kwak HS. 2009. CTX-M-14 and CTX-M-15 enzymes are the dominant type of extended-spectrum beta-lactamase in clinical isolates of Escherichia coli from Korea. J. Med. Microbiol. 58: 261-266. DOI ScienceOn
10	Valverde A, Canton R, Garcillan-Barcia MP, Novais A, Galan JC, Alvarado A, et al. 2009. Spread of bla(CTX-M-14) is driven mainly by IncK plasmids disseminated among Escherichia coli phylogroups A, B1, and D in Spain. Antimicrob. Agents Chemother. 53: 5204-5212. DOI ScienceOn
11	Huang DB, Mohamed JA, Nataro JP, DuPont HL, Jiang ZD, Okhuysen PC. 2007. Virulence characteristics and the molecular epidemiology of enteroaggregative Escherichia coli isolates from travellers to developing countries. J. Med. Microbiol. 56: 1386-1392. DOI
12	Hyma KE, Lacher DW, Nelson AM, Bumbaugh AC, Janda JM, Strockbine NA, et al. 2005. Evolutionary genetics of a new pathogenic Escherichia species: Escherichia albertii and related Shigella boydii strains. J. Bacteriol. 187: 619-628. DOI ScienceOn
13	Kaper JB, Nataro JP, Mobley HL. 2004. Pathogenic Escherichia coli. Nat. Rev. Microbiol. 2: 123-140. DOI ScienceOn
14	Kariuki S, Corkill JE, Revathi G, Musoke R, Hart CA. 2001. Molecular characterization of a novel plasmid-encoded cefotaximase (CTX-M-12) found in clinical Klebsiella pneumoniae isolates from Kenya. Antimicrob. Agents Chemother. 45: 2141-2143. DOI ScienceOn
15	Kim JY, Jeon SM, Rhie HG, Lee BK, Park MS, Lee H, et al. 2009. Rapid detection of extended spectrum $\beta$ -lactamase (ESBL) for Enterobacteriaceae by use of a multiplex PCRbased method. Infect. Chemother. 41: 181-184. DOI
16	Kim S, Kim J, Kang Y, Park Y, Lee B. 2004. Occurrence of extended-spectrum beta-lactamases in members of the genus Shigella in the Republic of Korea. J. Clin. Microbiol. 42: 5264-5269. DOI ScienceOn
17	Lee SG, Jeong SH, Lee H, Kim CK, Lee Y, Koh E, et al. 2009. Spread of CTX-M-type extended-spectrum beta-lactamases among bloodstream isolates of Escherichia coli and Klebsiella pneumoniae from a Korean hospital. Diagn. Microbiol. Infect. Dis. 63: 76-80. DOI ScienceOn
18	Bae IK, Lee YN, Jeong SH, Lee K, Lee H, Kwak HS, Woo GJ. 2007. High prevalence of SHV-12 and the emergence of CTXM- 12 in clinical isolates of Klebsiella pneumoniae from Korea. Int. J. Antimicrob. Agents 29: 362-364. DOI ScienceOn
19	Carattoli A, Bertini A, Villa L, Falbo V, Hopkins KL, Threlfall EJ. 2005. Identification of plasmids by PCR-based replicon typing. J. Microbiol. Methods 63: 219-228. DOI ScienceOn
20	Canton R, Gonzalez-Alba JM, Galan JC. 2012. CTX-M enzymes: origin and diffusion. Front. Microbiol. 3: 110.
21	CLSI. 2012. Performance Standards for Antimicrobial Susceptibility Testing; 22nd informational supplement (M100-S22). Wayne, PA.
22	Cottell JL, Webber MA, Coldham NG, Taylor DL, Cerdeno- Tarraga AM, Hauser H, et al. 2011. Complete sequence and molecular epidemiology of IncK epidemic plasmid encoding blaCTX-M-14. Emerg. Infect. Dis. 17: 645-652. DOI
23	Dhanji H, Khan P, Cottell JL, Piddock LJ, Zhang J, Livermore DM, Woodford N. 2012. Dissemination of pCTlike IncK plasmids harboring CTX-M-14 extended-spectrum beta-lactamase among clinical Escherichia coli isolates in the United Kingdom. Antimicrob. Agents Chemother. 56: 3376- 3377. DOI
24	Bae IK, Lee BH, Hwang HY, Jeong SH, Hong SG, Chang CL, et al. 2006. A novel ceftazidime-hydrolysing extendedspectrum beta-lactamase, CTX-M-54, with a single amino acid substitution at position 167 in the omega loop. J. Antimicrob. Chemother. 58: 315-319. DOI ScienceOn
25	Bae IK, Lee YN, Hwang HY, Jeong SH, Lee SJ, Kwak HS, et al. 2006. Emergence of CTX-M-12 extended-spectrum betalactamase- producing Escherichia coli in Korea. J. Antimicrob. Chemother. 58: 1257-1259. DOI ScienceOn
26	Fischer Walker CL, Sack D, Black RE. 2010. Etiology of diarrhea in older children, adolescents and adults: a systematic review. PLoS Negl. Trop. Dis. 4: e768. DOI

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