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http://dx.doi.org/10.4014/jmb.1306.06054

Biotransformation of Rosamicin Antibiotic into 10,11-Dihydrorosamicin with Enhanced In Vitro Antibacterial Activity Against MRSA  

Nguyen, Lan Huong (Department of Pharmaceutical Engineering, SunMoon University)
Nguyen, Huu Hoang (Department of Pharmaceutical Engineering, SunMoon University)
Shrestha, Anil (Department of Pharmaceutical Engineering, SunMoon University)
Sohng, Jae Kyung (Department of Pharmaceutical Engineering, SunMoon University)
Yoon, Yeo Joon (Department of Chemistry and Nano Science, Ewha Womans University)
Park, Je Won (Department of Pharmaceutical Engineering, SunMoon University)
Publication Information
Journal of Microbiology and Biotechnology / v.24, no.1, 2014 , pp. 44-47 More about this Journal
Abstract
A biotransformation approach using microbes as biocatalysts can be an efficient tool for the targeted modification of existing antibiotic chemical scaffolds to create previously uncharacterized therapeutic agents. By employing a recombinant Streptomyces venezuelae strain as a microbial catalyst, a reduced macrolide, 10,11-dihydrorosamicin, was created from rosamicin macrolide. Its chemical structure was spectroscopically elucidated, and the new rosamicin analog showed 2-4-fold higher antibacterial activity against two strains of methicillin-resistant Staphylococcus aureus compared with its parent rosamicin. This kind of biocatalytic approach is able to expand existing antibiotic entities and can also provide more diverse therapeutic resources.
Keywords
Enhanced anti-MRSA activity; microbial catalyst; reduced macrolide antibiotic; Streptomyces venezuelae; 10,11-dihydrorosamicin;
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