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http://dx.doi.org/10.4014/jmb.1404.04024

Development of a Single Nucleotide Polymorphism DNA Microarray for the Detection and Genotyping of the SARS Coronavirus  

Guo, Xi (TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA)
Geng, Peng (School of Basic Medical Sciences, Tianjin Medical University)
Wang, Quan (School of Basic Medical Sciences, Tianjin Medical University)
Cao, Boyang (TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA)
Liu, Bin (TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA)
Publication Information
Journal of Microbiology and Biotechnology / v.24, no.10, 2014 , pp. 1445-1454 More about this Journal
Abstract
Severe acute respiratory syndrome (SARS), a disease that spread widely in the world during late 2002 to 2004, severely threatened public health. Although there have been no reported infections since 2004, the extremely pathogenic SARS coronavirus (SARS-CoV), as the causative agent of SARS, has recently been identified in animals, showing the potential for the re-emergence of this disease. Previous studies showed that 27 single nucleotide polymorphism (SNP) mutations among the spike (S) gene of this virus are correlated closely with the SARS pathogenicity and epidemicity. We have developed a SNP DNA microarray in order to detect and genotype these SNPs, and to obtain related information on the pathogenicity and epidemicity of a given strain. The microarray was hybridized with PCR products amplified from cDNAs obtained from different SARS-CoV strains. We were able to detect 24 SNPs and determine the type of a given strain. The hybridization profile showed that 19 samples were detected and genotyped correctly by using our microarray, with 100% accuracy. Our microarray provides a novel method for the detection and epidemiological surveillance of SARS-CoV.
Keywords
SARS; SARS-CoV; S gene; SNP; DNA microarray;
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