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http://dx.doi.org/10.4014/jmb.1212.12042

Performance of the xTAG$^{(R)}$ Gastrointestinal Pathogen Panel, a Multiplex Molecular Assay for Simultaneous Detection of Bacterial, Viral, and Parasitic Causes of Infectious Gastroenteritis  

Claas, Eric C. (Department of Medical Microbiology, Leiden University Medical Center)
Burnham, Carey-Ann D. (Departments of Pathology and Immunology and Pediatrics, Washington University School of Medicine)
Mazzulli, Tony (Department of Microbiology, University Health Network/Mount Sinai Hospital, University of Toronto)
Templeton, Kate (Specialist Virology Centre, Royal Infirmary of Edinburgh)
Topin, Francois (Luminex B.V.)
Publication Information
Journal of Microbiology and Biotechnology / v.23, no.7, 2013 , pp. 1041-1045 More about this Journal
Abstract
The xTAG$^{(R)}$ Gastrointestinal Pathogen Panel (GPP) is a multiplexed molecular test for 15 gastrointestinal pathogens. The sensitivity and specificity of this test were assessed in 901 stool specimens collected from pediatric and adult patients at four clinical sites. A combination of conventional and molecular methods was used as comparator. Sensitivity could be determined for 12 of 15 pathogens and was 94.3% overall. The specificity across all 15 targets was 98.5%. Testing for the pathogen identified was not requested by the physician in 65% of specimens. The simultaneous detection of these 15 pathogens can provide physicians with a more comprehensive assessment of the etiology of diarrheal disease.
Keywords
Luminex; xTAG GPP; multiplex; gastroenteritis; infectious diarrhea;
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