Browse > Article
http://dx.doi.org/10.4014/jmb.1211.11080

Repression of Type-1 Fimbriae in Shiga Toxin-Producing Escherichia coli O91:H21 Isolated from Asymptomatic Human Carriers in Korea  

Kim, Jung-Beom (Division of Health Research and Planning, Gyeonggi-do Research Institute of Health and Environment)
Oh, Kyung-Hwan (Division of Enteric Bacterial Infections, Center for Infectious Diseases, Korea National Institute of Health)
Park, Mi-Sun (Division of Enteric Bacterial Infections, Center for Infectious Diseases, Korea National Institute of Health)
Cho, Seung-Hak (Division of Enteric Bacterial Infections, Center for Infectious Diseases, Korea National Institute of Health)
Publication Information
Journal of Microbiology and Biotechnology / v.23, no.5, 2013 , pp. 731-737 More about this Journal
Abstract
Seventy-four Shiga toxin-producing Escherichia coli (STEC) isolates belonging to the serotype O91:H21 were isolated from 1,643 asymptomatic human carriers in a STEC outbreak at Gwangju in Korea. Although the isolates did not cause any symptoms, all of them produced Shiga toxins 1 (Stx1) and 2 (Stx2). In order to determine why these strains cause no symptoms, we explored the differences in virulence potential between the asymptomatic STEC O91:H21 isolates and symptomatic STEC O91:H21 strains (ATCC 51435 and ATCC 51434). The asymptomatic STEC O91:H21 isolates showed strongly reduced cytopathic effects compared with the symptomatic strains when intact bacterial cells were used as an inoculant. Moreover, we found a reduced adherence phenotype when testing asymptomatic strains on HeLa cells. Real-time quantitative PCR results suggest that transcriptional repression of the genes encoding type-1 fimbriae occurs in the asymptomatic isolates but not in the symptomatic strains.
Keywords
Shiga toxin-producing Escherichia coli (STEC); asymptomatic human carriers; type 1 fimbriae;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Coombes, B. K., M. E. Wickham, M. Mascarenhas, S. Gruenheid, B. B. Finlay, and M. A. Karmali. 2008. Molecular analysis as an aid to assess the public health risk of non-O157 Shiga toxin-producing Escherichia coli strains. Appl. Environ. Microbiol. 74: 2153-2160.   DOI   ScienceOn
2 Backhed, F., B. Alsen, N. Roche, J. Angstrom, A. von Euler, M. E. Breimer, et al. 2002. Identification of target tissue glycosphingolipid receptors for uropathogenic, F1C-fimbriated Escherichia coli and its role in mucosal inflammation. J. Biol. Chem. 277: 18198-18205.   DOI   ScienceOn
3 Caprioli, A., S. Morabito, H. Brugere, and E. Oswald. 2005. Enterohaemorrhagic Escherichia coli: Emerging issues on virulence and modes of transmission. Vet. Res. 36: 289-311.   DOI   ScienceOn
4 Clarke, S. C., R. D. Haigh, P. P. E. Freestone, and P. H. Williams. 2003. Virulence of enteropathogenic Escherichia coli, a global pathogen. Clin. Microbiol. Rev. 16: 365-378.   DOI   ScienceOn
5 Fitzhenry, R. J., D. J. Pickard, E. L. Hartland, S. Reece, G. Dougan, A. D. Phillips, et al. 2002. Intimin type influences the site of human intestinal mucosal colonisation by enterohaemorrhagic Escherichia coli O157:H7. J. Infect. Dis. 50: 180-185.
6 Friedrich, A. W., M. Bielaszewska, W. L. Zhang, M. Pulz, T. Kuczius, A. Ammon, et al. 2002. Escherichia coli harboring Shiga toxin 2 gene variants: Frequency and association with clinical symptoms. J. Infect. Dis. 185: 74-84.   DOI   ScienceOn
7 Lindgren, S. W., A. R. Melton, and A. D. O'Brien. 1993. Virulence of enterohemorrhagic Escherichia coli O91:H21 clinical isolates in an orally infected mouse model. Infect. Immun. 61: 3832-3842.
8 Garmendia, J., G. Frankel, and V. F. Crepin. 2005. Enteropathogenic and enterohemorrhagic Escherichia coli infections: Translocation, translocation, translocation. Infect. Immun. 73: 2573-2585.   DOI   ScienceOn
9 Jores, J., L. Rumer, and L. H. Wieler. 2004. Impact of the locus of enterocyte effacement pathogenicity island on the evolution of pathogenic Escherichia coli. Int. J. Med. Microbiol. 294: 103-113.   DOI   ScienceOn
10 Klemm, P., V. Roos, G. C. Ulett, C. Svanborg, and M. A. Schembri. 2006. Molecular characterization of the Escherichia coli asymptomatic bacteriuria strain 83972: The taming of a pathogen. Infect. Immun. 74: 781-785.   DOI   ScienceOn
11 Lu, Y., S. Iyoda, H. Satou, H. Satou, K. Itoh, T. Saitoh, et al. 2006. A new immunoglobulin-binding protein, EibG, is responsible for the chain-like adhesion phenotype of locus of enterocyte effacement-negative, Shiga toxin-producing Escherichia coli. Infect. Immun. 74: 5747-5755.   DOI   ScienceOn
12 Makino, S. I., T. Tobe, H. Asakura, M. Watarai, T. Ikeda, K. Takeshi, and C. Sasakawa. 2003. Distribution of the secondary type III secretion system locus found in enterohemorrhagic Escherichia coli O157:H7 isolates among Shiga toxin-producing E. coli strains. J. Clin. Microbiol. 41: 2341-2347.   DOI   ScienceOn
13 Melton-Celsa, A. R., J. F. Kokai-Kun, and A. D. O'Brien. 2002. Activation of Shiga toxin type 2d (Stx2d) by elastase involves cleavage of the C-terminal two amino acids of the A2 peptide in the context of the appropriate B pentamer. Mol. Microbiol. 43: 207-215.   DOI   ScienceOn
14 Mukherjee, J., K. Chios, D. Fishwild, D. Hudson, S. O'Donnell, S. M. Rich, et al. 2002. Human Stx2-specific monoclonal antibodies prevent systemic complications of Escherichia coli O157:H7 infection. Infect. Immun. 70: 612-619.   DOI   ScienceOn
15 Roos, V. and P. Klemm. 2006. Global gene expression profiling of the asymptomatic bacteriuria Escherichia coli strain 83972 in the human urinary tract. Infect. Immun. 74: 3565-3575.   DOI   ScienceOn
16 Muniesa, M., J. Recktenwald, M. Bielaszewska, H. Karch, and H. Schmidt. 2000. Characterization of a Shiga toxin 2e-converting bacteriophage from an Escherichia coli strain of human origin. Infect. Immun. 68: 4850-4855.   DOI
17 Nataro, J. P. and J. B. Kaper. 1998. Diarrheagenic Escherichia coli. Clin. Microbiol. Rev. 11: 142-201.
18 Park, S. H., S. H. Kim, J. J. Seo, H. Y. Kee, M. J. Kim, K. W. Seo, et al. 2006. An outbreak of inapparent non-O157 enterohemorrhagic Escherichia coli infection. Korean J. Intern. Med. 70: 495-504.
19 Roos, V., M. A. Schembri, G. C. Ulett, and P. Klemm. 2006. Asymptomatic bacteriuria Escherichia coli strain 83972 carries mutations in the foc locus and is unable to express F1C fimbriae. Microbiology 152: 1799-1806.   DOI   ScienceOn
20 Roos, V., G. C. Ulett, M. A. Schembri, and P. Klemm. 2006. The asymptomatic bacteriuria Escherichia coli strain 83972 outcompetes uropathogenic E. coli strains in human urine. Infect. Immun. 74: 615-624.   DOI   ScienceOn
21 Stephan, R. and F. Untermann. 1999. Virulence factors and phenotypical traits of verotoxin-producing Escherichia coli strains isolated from asymptomatic human carriers. J. Clin. Microbiol. 37: 1570-1572.
22 Toma, C., N. Higa, S. Iyoda, M. Rivas, and M. Iwanaga. 2006. The long polar fimbriae genes identified in Shiga toxin-producing Escherichia coli are present in other diarrheagenic E. coli and in the standard E. coli collection of reference (ECOR) strains. Res. Microbiol. 157: 153-161.   DOI   ScienceOn
23 Torres, A. G., K. J. Kanack, C. B. Tutt, V. Popov, and J. B. Kaper. 2004. Characterization of the second long polar (LP) fimbriae of Escherichia coli O157:H7 and distribution of LP fimbriae in other pathogenic E. coli strains. FEMS Microbiol. Lett. 238: 333-344.
24 Zhang, W., B. Kohler, E. Oswald, L. Beutin, H. Karch, S. Morabito, et al. 2002. Genetic diversity of intimin genes of attaching and effacing Escherichia coli strains. J. Clin. Microbiol. 40: 4486-4492.   DOI   ScienceOn