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http://dx.doi.org/10.4014/jmb.1302.02017

Selective Inhibition of Ammonia Oxidation and Nitrite Oxidation Linked to $N_2O$ Emission with Activated Sludge and Enriched Nitrifiers  

Ali, Toor Umair (Department of Environmental Sciences and Biotechnology, Hallym University)
Kim, Minwook (Department of Environmental Sciences and Biotechnology, Hallym University)
Kim, Dong-Jin (Department of Environmental Sciences and Biotechnology, Hallym University)
Publication Information
Journal of Microbiology and Biotechnology / v.23, no.5, 2013 , pp. 719-723 More about this Journal
Abstract
Nitrification in wastewater treatment emits a significant amount of nitrous oxide ($N_2O$), which is one of the major greenhouse gases. However, the actual mechanism or metabolic pathway is still largely unknown. Selective nitrification inhibitors were used to determine the nitrification steps responsible for $N_2O$ emission with activated sludge and enriched nitrifiers. Allylthiourea (86 ${\mu}M$) completely inhibited ammonia oxidation and $N_2O$ emission both in activated sludge and enriched nitrifiers. Sodium azide (24 ${\mu}M$) selectively inhibited nitrite oxidation and it led to more $N_2O$ emission than the control experiment both in activated sludge and enriched nitrifiers. The inhibition tests showed that $N_2O$ emission was mainly related to the activity of ammonia oxidizers in aerobic condition, and the inhibition of ammonia monooxygenase completely blocked $N_2O$ emission. On the other hand, $N_2O$ emission increased significantly as the nitrogen flux from nitrite to nitrate was blocked by the selective inhibition of nitrite oxidation.
Keywords
Activated sludge; ammonia oxidation; enriched nitrifiers; inhibition; nitrite oxidation; $N_2O$ emission;
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