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http://dx.doi.org/10.4014/jmb.1211.11012

Production of Ginsenoside Rd from Ginsenoside Rc by ${\alpha}-{\small{L}}$-Arabinofuranosidase from Caldicellulosiruptor saccharolyticus  

Shin, Kyung-Chul (Department of Bioscience and Biotechnology, Konkuk University)
Lee, Gi-Woong (Department of Bioscience and Biotechnology, Konkuk University)
Oh, Deok-Kun (Department of Bioscience and Biotechnology, Konkuk University)
Publication Information
Journal of Microbiology and Biotechnology / v.23, no.4, 2013 , pp. 483-488 More about this Journal
Abstract
Ginsenoside Rd was produced from ginsenoside Rc using a thermostable recombinant ${\alpha}-{\small{L}}$-arabinofuranosidase from Caldicellulosiruptor saccharolyticus. The optimal reaction conditions for the production of ginsenoside Rd from Rc were pH 5.5, $80^{\circ}C$, 227 U enzyme/ml, and 8.0 g/l ginsenoside Rc in the presence of 30% (v/v) n-hexane. Under these conditions, the enzyme produced 7.0 g/l ginsenoside Rd after 30 min, with a molar yield of 100% and a productivity of 14 g $l^{-1}\;h^{-1}$. The conversion yield and productivity of ginsenoside Rd are the highest reported thus far among enzymatic transformations.
Keywords
Ginsenoside Rd; ginsenoside Rc; biotransformation; ${\alpha}-{\small{L}}$-arabinofuranosidase; Caldicellulosiruptor saccharolyticus;
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