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http://dx.doi.org/10.4014/jmb.1207.09020

Characterization of a Blend-Biosurfactant of Glycolipid and Lipopeptide Produced by Bacillus subtilis TU2 Isolated from Underground Oil-Extraction Wastewater  

Cheng, Fangyu (Department of Chemical Engineering, Tsinghua University)
Tang, Cheng (Department of Chemical Engineering, Tsinghua University)
Yang, Huan (Department of Chemical Engineering, Tsinghua University)
Yu, Huimin (Department of Chemical Engineering, Tsinghua University)
Chen, Yu (Department of Chemical Engineering, Tsinghua University)
Shen, Zhongyao (Department of Chemical Engineering, Tsinghua University)
Publication Information
Journal of Microbiology and Biotechnology / v.23, no.3, 2013 , pp. 390-396 More about this Journal
Abstract
Biosurfactants have versatile properties and potential industrial applications. A new producer, B. subtilis TU2, was isolated from the underground oil-extraction wastewater of Shengli Oilfield, China. Preliminary flask culture showed that the titer of biosurfactant obtained from the broth of TU2 was ~1.5 g/l at 48 h (718 mg/l after purification), with a reduced surface tension of 32.5 mN/m. The critical micelle concentration was measured as 50 mg/l and the surface tension maintained stability in solution with 50 g/l NaCl and 16 g/l $CaCl_2$ after 5 days of incubation at $70^{\circ}C$. FT-IR spectra exhibited the structure information of both glycolipid and lipopeptide. MALDI-TOF-MS analyses confirmed that the biosurfactant produced by B. subtilis TU2 was a blend of glycolipid and lipopeptide, including rhamnolipid, surfactin, and fengycin. The blended biosurfactant showed 86% of oil-washing efficiency and fine emulsification activity on crude oil, suggesting its potential application in enhanced oil recovery.
Keywords
Biosurfactant; oil-extraction wastewater; glycolipid; lipopeptide; enhanced oil recovery (EOR);
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