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http://dx.doi.org/10.4014/jmb.1207.07061

Cyt1Aa from Bacillus thuringiensis subsp. israelensis Enhances Mosquitocidal Activity of B. thuringiensis subsp. kurstaki HD-1 Against Aedes aegypti but not Culex quinquefasciatus  

Park, Hyun-Woo (Department of Natural and Mathematical Sciences, California Baptist University)
Pino, Brent C. (Department of Natural and Mathematical Sciences, California Baptist University)
Kozervanich-Chong, Switzerlyna (Department of Health Science, California Baptist University)
Hafkenscheid, Erika A. (School of Nursing, California Baptist University)
Oliverio, Ryan M. (Department of Natural and Mathematical Sciences, California Baptist University)
Federici, Brian A. (Department of Entomology, University of California)
Bideshi, Dennis K. (Department of Natural and Mathematical Sciences, California Baptist University)
Publication Information
Journal of Microbiology and Biotechnology / v.23, no.1, 2013 , pp. 88-91 More about this Journal
Abstract
The Cyt1Aa protein of Bacillus thuringiensis subsp. israelensis is known to synergize mosquitocidal proteins of B. thuringiensis and Bacillus sphaericus strains. Cyt1Aa is highly lipophilic, and after binding in vivo to the midgut microvillar membrane serves as a "receptor" for mosquitocidal Cry proteins, which subsequently form cation channels that kill mosquito larvae. Here we report that Cyt1Aa can serve a similar function for lepidopteran-specific Cry proteins of B. thuringiensis in certain mosquito larvae. Engineering Cyt1Aa into the HD-1 isolate of B. thuringiensis subsp. kurstaki enhanced toxicity against $4^{th}$ instars of Aedes aegypti, but not against $4^{th}$ instars of Culex quinquefasciatus.
Keywords
Cyt1Aa; Bacillus thuringiensis subsp. kurstaki HD-1; Aedes aegypti; synergism;
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