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http://dx.doi.org/10.4014/jmb.1106.05062

Identification of Reassortant Pandemic H1N1 Influenza Virus in Korean Pigs  

Han, Jae-Yeon (Department of Veterinary Medicine Virology Laboratory College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University)
Park, Sung-Jun (Department of Veterinary Medicine Virology Laboratory College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University)
Kim, Hye-Kwon (Department of Veterinary Medicine Virology Laboratory College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University)
Rho, Se-Mi (Department of Veterinary Medicine Virology Laboratory College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University)
Nguyen, Giap Van (Department of Veterinary Medicine Virology Laboratory College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University)
Song, Dae-Sub (Research Unit, Green Cross Veterinary Products)
Kang, Bo-Kyu (Research Unit, Green Cross Veterinary Products)
Moon, Hyung-Jun (Research Unit, Green Cross Veterinary Products)
Yeom, Min-Joo (Research Unit, Green Cross Veterinary Products)
Park, Bong-Kyun (Department of Veterinary Medicine Virology Laboratory College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University)
Publication Information
Journal of Microbiology and Biotechnology / v.22, no.5, 2012 , pp. 699-707 More about this Journal
Abstract
Since the 2009 pandemic human H1N1 influenza A virus emerged in April 2009, novel reassortant strains have been identified throughout the world. This paper describes the detection and isolation of reassortant strains associated with human pandemic influenza H1N1 and swine influenza H1N2 (SIV) viruses in swine populations in South Korea. Two influenza H1N2 reassortants were detected, and subtyped by PCR. The strains were isolated using Madin-Darby canine kidney (MDCK) cells, and genetically characterized by phylogenetic analysis for genetic diversity. They consisted of human, avian, and swine virus genes that were originated from the 2009 pandemic H1N1 virus and a neuraminidase (NA) gene from H1N2 SIV previously isolated in North America. This identification of reassortment events in swine farms raises concern that reassortant strains may continuously circulate within swine populations, calling for the further study and surveillance of pandemic H1N1 among swine.
Keywords
H1N2 influenza A virus; pandemic (H1N1) 2009 influenza A virus; reassortment; South Korea; swine;
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1 Sreta, D., S. Tantawet, S. N. Na Ayudhya, A. Thontiravong, M. Wongphatcharachai, J. Lapkuntod, et al. 2010. Pandemic (H1N1) 2009 virus on commercial swine farm, Thailand. Emerg. Infect. Dis. 16: 1587-1590.   DOI
2 Starick, E., E. Lange, S. Fereidouni, C. Bunzenthal, R. Hoveler, A. Kuczka, et al. 2011. Reassorted pandemic (H1N1) 2009 influenza A virus discovered from pigs in Germany. J. Gen. Virol. 92: 1184-1188.   DOI   ScienceOn
3 Thompson, J. D., T. J. Gibson, F. Plewniak, F. Jeanmougin, and D. G. Higgins. 1997. The CLUSTAL_X Windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25: 4876-4882.   DOI   ScienceOn
4 Tobita, K., A. Sugiura, C. Enomote, and M. Furuyama. 1975. Plaque assay and primary isolation of influenza A viruses in an established line of canine kidney cells (MDCK) in the presence of trypsin. Med. Microbiol. Immunol. 162: 9-14.   DOI   ScienceOn
5 Vijaykrishna, D., L. L. Poon, H. C. Zhu, S. K. Ma, O. T. Li, C. L. Cheung, et al. 2010. Reassortment of pandemic H1N1/2009 influenza A virus in swine. Science 328: 1529.   DOI   ScienceOn
6 Webster, R. G., W. J. Bean, O. T. Gorman, T. M. Chambers, and Y. Kawaoka. 1992. Evolution and ecology of influenza A viruses. Microbiol. Rev. 56: 152-179.
7 Jung, K. and C. Chae. 2004. Phylogenetic analysis of an H1N2 influenza A virus isolated from a pig in Korea. Brief Report. Arch. Virol. 149: 1415-1422.
8 Jung, K. and C. Chae. 2005. First outbreak of respiratory disease associated with swine influenza H1N2 virus in pigs in Korea. J. Vet. Diagn. Invest. 17: 176-178.   DOI   ScienceOn
9 Kitikoon, P., D. Sreta, S. N. Na Ayudhya, M. Wongphatcharachai, J. Lapkuntod, D. Prakairungnamthip, et al. 2011. Brief report: Molecular characterization of a novel reassorted pandemic H1N1 2009 in Thai pigs. Virus Genes 43: 1-5.   DOI   ScienceOn
10 Lee, C. S., B. K. Kang, H. K. Kim, S. J. Park, B. K. Park, K. Jung, and D. S. Song. 2008. Phylogenetic analysis of swine influenza viruses recently isolated in Korea. Virus Genes 37: 168-176.   DOI   ScienceOn
11 Lee, C. S., B. K. Kang, D. H. Lee, S. H. Lyou, B. K. Park, S. K. Ann, et al. 2008. One-step multiplex RT-PCR for detection and subtyping of swine influenza H1, H3, N1, N2 viruses in clinical samples using a dual priming oligonucleotide (DPO) system. J. Virol. Methods 151: 30-34.   DOI   ScienceOn
12 Luoh, S. M., M. W. McGregor, and V. S. Hinshaw. 1992. Hemagglutinin mutations related to antigenic variation in H1 swine influenza viruses. J. Virol. 66: 1066-1073.
13 Massin, P., G. Kuntz-Simon, C. Barbezange, C. Deblanc, A. Oger, E. Marquet-Blouin, et al. 2010. Temperature sensitivity on growth and/or replication of H1N1, H1N2 and H3N2 influenza A viruses isolated from pigs and birds in mammalian cells. Vet. Microbiol. 142: 232-241.   DOI   ScienceOn
14 Moreno, A., L. Di Trani, S. Faccini, G. Vaccari, D. Nigrelli, M. B. Boniotti, et al. 2010. Novel H1N2 swine influenza reassortant strain in pigs derived from the pandemic H1N1/2009 virus. Vet. Microbiol. 149: 472-477.
15 Yu, H., P. C. Zhang, Y. J. Zhou, G. X. Li, J. Pan, L. P. Yan, et al. 2009. Isolation and genetic characterization of avian-like H1N1 and novel ressortant H1N2 influenza viruses from pigs in China. Biochem. Biophys Res. Commun. 386: 278-283.   DOI   ScienceOn
16 Weingartl, H. M., Y. Berhane, T. Hisanaga, J. Neufeld, H. Kehler, C. Emburry-Hyatt, et al. 2010. Genetic and pathobiologic characterization of pandemic H1N1 2009 influenza viruses from a naturally infected swine herd. J. Virol. 84: 2245-2256.   DOI   ScienceOn
17 WHO. 2009. Current WHO Phase of Pandemic Alert.
18 Xu, C., Q. Zhu, H. Yang, X. Zhang, C. Qiao, Y. Chen, et al. 2009. Two genotypes of H1N2 swine influenza viruses appeared among pigs in China. J. Clin. Virol. 46: 192-195.   DOI   ScienceOn
19 Zhou, N. N., D. A. Senne, J. S. Landgraf, S. L. Swenson, G. Erickson, K. Rossow, et al. 1999. Genetic reassortment of avian, swine, and human influenza A viruses in American pigs. J. Virol. 73: 8851-8856.
20 Zou, S. 1997. A practical approach to genetic screening for influenza virus variants. J. Clin. Microbiol. 35: 2623-2627.
21 Brown, I. H., P. Chakraverty, P. A. Harris, and D. J. Alexander. 1995. Disease outbreaks in pigs in Great Britain due to an influenza A virus of H1N2 subtype. Vet. Rec. 136: 328-329.   DOI   ScienceOn
22 Brown, I. H., P. A. Harris, J. W. McCauley, and D. J. Alexander. 1998. Multiple genetic reassortment of avian and human influenza A viruses in European pigs, resulting in the emergence of an H1N2 virus of novel genotype. J. Gen. Virol. 79: 2947-2955.
23 Chen, G. W. and S. R. Shih. 2009. Genomic signatures of influenza A pandemic (H1N1) 2009 virus. Emerg. Infect. Dis. 15: 1897-1903.   DOI
24 Pereda, A., J. Cappuccio, M. A. Quiroga, E. Baumeister, L. Insarralde, M. Ibar, et al. 2010. Pandemic (H1N1) 2009 outbreak on pig farm, Argentina. Emerg. Infect. Dis. 16: 304-307.   DOI
25 Olsen, C. W. 2002. The emergence of novel swine influenza viruses in North America. Virus Res. 85: 199-210.   DOI   ScienceOn
26 Olsen, C. W., I. H. Brown, B. C. Easterday, and V. Reeth. 2006. In B. E. Straw, J. J. Zimmerman, S. D'Allaire, and D. J. Taylor (eds.). Disease of Swine. The Blackwell Publishing Professinal.
27 Pascua, P. N., M. S. Song, J. H. Lee, H. W. Choi, J. H. Han, J. H. Kim, et al. 2008. Seroprevalence and genetic evolutions of swine influenza viruses under vaccination pressure in Korean swine herds. Virus Res. 138: 43-49.   DOI   ScienceOn
28 Schnitzler, S. U. and P. Schnitzler. 2009. An update on swineorigin influenza virus A/H1N1: A review. Virus Genes 39: 279-292.   DOI   ScienceOn
29 Shaw, M. W., N. H. Arden, and H. F. Maassab. 1992. New aspects of influenza viruses. Clin. Microbiol. Rev. 5: 74-92.
30 Shi, W., F. Lei, C. Zhu, F. Sievers, and D. G. Higgins. 2010. A complete analysis of HA and NA genes of influenza A viruses. PLoS One 5: e14454.   DOI   ScienceOn
31 Song, D. S., C. S. Lee, K. Jung, B. K. Kang, J. S. Oh, Y. D. Yoon, et al. 2007. Isolation and phylogenetic analysis of H1N1 swine influenza virus isolated in Korea. Virus Res. 125: 98-103.   DOI   ScienceOn
32 Song, D. S., J. Y. Lee, J. S. Oh, K. S. Lyoo, K. J. Yoon, Y. H. Park, and B. K. Park. 2003. Isolation of H3N2 swine influenza virus in South Korea. J. Vet. Diagn. Invest. 15: 30-34.   DOI   ScienceOn
33 Song, M. S., J. H. Lee, P. N. Pascua, Y. H. Baek, H. I. Kwon, K. J. Park, et al. 2009. Evidence of human-to-swine transmission of the pandemic (H1N1) 2009 influenza virus in South Korea. J. Clin. Microbiol. 48: 3204-3211.
34 Alexander, D. J. 2007. An overview of the epidemiology of avian influenza. Vaccine 25: 5637-5644.   DOI   ScienceOn
35 Choi, Y. K., S. M. Goyal, M. W. Farnham, and H. S. Joo. 2002. Phylogenetic analysis of H1N2 isolates of influenza A virus from pigs in the United States. Virus Res. 87: 173-179.   DOI   ScienceOn
36 Claas, E. C., Y. Kawaoka, J. C. de Jong, N. Masurel, and R. G. Webster. 1994. Infection of children with avian-human reassortant influenza virus from pigs in Europe. Virology 204: 453-457.   DOI   ScienceOn
37 Fouchier, R. A., V. Munster, A. Wallensten, T. M. Bestebroer, S. Herfst, D. Smith, et al. 2005. Characterization of a novel influenza A virus hemagglutinin subtype (H16) obtained from black-headed gulls. J. Virol. 79: 2814-2822.   DOI   ScienceOn
38 Brown, I. H. 2000. The epidemiology and evolution of influenza viruses in pigs. Vet. Microbiol. 74: 29-46.   DOI   ScienceOn
39 Garten, R. J., C. T. Davis, C. A. Russell, B. Shu, S. Lindstrom, A. Balish, et al. 2009. Antigenic and genetic characteristics of swine-origin 2009 A(H1N1) influenza viruses circulating in humans. Science 325: 197-201.   DOI   ScienceOn
40 Howard, W. A., S. C. Essen, B. W. Strugnell, C. Russell, L. Barass, S. M. Reid, and I. H. Brown. 2011. Reassortant pandemic (H1N1) 2009 virus in pigs, United Kingdom. Emerg. Infect. Dis. 17: 1049-1052.   DOI
41 Ito, T., J. N. Couceiro, S. Kelm, L. G. Baum, S. Krauss, M. R. Castrucci, et al. 1998. Molecular basis for the generation in pigs of influenza A viruses with pandemic potential. J. Virol. 72: 7367-7373.
42 Jo, S. K., H. S. Kim, S. W. Cho, and S. H. Seo. 2007. Genetic and antigenic characterization of swine H1N2 influenza viruses isolated from Korean pigs. J. Microbiol. Biotechnol. 17: 868-872.