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http://dx.doi.org/10.4014/jmb.1111.11005

Evaluation of a Pretreatment Method for Two-Dimensional Gel Electrophoresis of Synovial Fluid Using Cartilage Oligomeric Matrix Protein as a Marker  

Kong, Min-Kyung (Department of Molecular Science and Technology, Ajou University)
Min, Byoung-Hyun (Department of Molecular Science and Technology, Ajou University)
Lee, Pyung-Cheon (Department of Molecular Science and Technology, Ajou University)
Publication Information
Journal of Microbiology and Biotechnology / v.22, no.5, 2012 , pp. 654-658 More about this Journal
Abstract
Osteoarthritis (OA) is the most common rheumatic pathology. One of the major objectives of OA research is the development of early diagnostic strategies such as those using proteomic technology. Synovial fluid (SF) in OA patients is a potential source of biomarkers for OA. The efficient and reliable preparation of SF proteomes is a critical step towards biomarker discovery. In this study, we have optimized a pretreatment method for twodimensional gel electrophoresis (2DE) separation of the SF proteome, by enriching low-abundance proteins and simultaneously removing hyaluronic acid, albumin, and IgG. SF samples pretreated using this optimized method were then evaluated by 1DE and 2DE separation followed by immunodetection of cartilage oligomeric matrix protein (COMP), a known OA biomarker, and by the identification of 3 proteins (apolipoprotein, haptoglobin precursor, and fibrinogen D fragment) that are related to joint diseases.
Keywords
Osteoarthritis; synovial fluid; two-dimensional gel electrophoresis; biomarker; cartilage oligomeric matrix protein; hyaluronic acid;
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1 Chang, X., J. Han, Y. Zhao, X. Yan, S. Sun, and Y. Cui. 2010. Increased expression of carbonic anhydrase I in the synovium of patients with ankylosing spondylitis. BMC Musculoskelet. Disord. 11: 279.   DOI   ScienceOn
2 De Ceuninck, F. and F. Berenbaum. 2009. Proteomics: Addressing the challenges of osteoarthritis. Drug Discov. Today 14: 661-667.   DOI   ScienceOn
3 Ghosh, P. 1994. The role of hyaluronic acid (hyaluronan) in health and disease: Interactions with cells, cartilage and components of synovial fluid. Clin. Exp. Rheumat. 12: 75-82.
4 Gibson, D. S. and M. E. Rooney. 2007. The human synovial fluid proteome: A key factor in the pathology of joint disease. Proteomics Clin. Appl. 1: 889-899.   DOI   ScienceOn
5 Kamphorst, J. J., R. Van Der Heijden, J. DeGroot, F. P. J. G. Lafeber, T. H. Reijmers, B. Van El, et al. 2007. Profiling of endogenous peptides in human synovial fluid by NanoLC-MS: Method validation and peptide identification. J. Proteome Res. 6: 4388-4396.   DOI   ScienceOn
6 Liao, H., J. Wu, E. Kuhn, W. Chin, B. Chang, M. D. Jones, et al. 2004. Use of mass spectrometry to identify protein biomarkers of disease severity in the synovial fluid and serum of patients with rheumatoid arthritis. Arthritis Rheum. 50: 3792-3803.   DOI   ScienceOn
7 Marshall, K. W., H. Zhang, and N. Nossova. 2006. Chondrocyte genomics: Implications for disease modification in osteoarthritis. Drug Discov. Today 11: 825-832.   DOI   ScienceOn
8 Morozzi, G., M. Fabbroni, F. Bellisai, G. Pucci, and M. Galeazzi. 2007. Cartilage oligomeric matrix protein level in rheumatic diseases: Potential use as a marker for measuring articular cartilage damage and/or the therapeutic efficacy of treatments. N.Y. Acad. Sci. 1108: 398-407.   DOI   ScienceOn
9 Patra, D. and L. J. Sandell. 2011. Recent advances in biomarkers in osteoarthritis. Curr. Opin. Rheumatol.
10 Ruiz-Romero, C. and F. J. Blanco. 2010. Proteomics role in the search for improved diagnosis, prognosis and treatment of osteoarthritis. Osteoarthr. Cartil. 18: 500-509.   DOI   ScienceOn
11 Ruiz-Romero, C., V. Calamia, V. Carreira, J. Mateos, P. Fernandez, and F. J. Blanco. 2010. Strategies to optimize two-dimensional gel electrophoresis analysis of the human joint proteome. Talanta 80: 1552-1560.   DOI   ScienceOn
12 Sinz, A., M. Bantscheff, S. Mikkat, B. Ringel, S. Drynda, J. Kekow, et al. 2002. Mass spectrometric proteome analyses of synovial fluids and plasmas from patients suffering from rheumatoid arthritis and comparison to reactive arthritis or osteoarthritis. Electrophoresis 23: 3445-3456.   DOI   ScienceOn
13 Skriner, K., K. Adolph, P. R. Jungblut, and G. R. Burmester. 2006. Association of citrullinated proteins with synovial exosomes. Arthritis Rheumat. 54: 3809-3814.   DOI   ScienceOn
14 Tseng, S., A. H. Reddi, and P. E. Di Cesare. 2009. Cartilage oligomeric matrix protein (COMP): A biomarker of arthritis. Biomark. Insights 2009: 33-44.
15 Williams, A., J. R. Smith, D. Allaway, P. Harris, S. Liddell, and A. Mobasheri. 2011. Applications of proteomics in cartilage biology and osteoarthritis research. Front. Biosci. 16: 2622-2641.   DOI   ScienceOn
16 Yamagiwa, H., G. Sarkar, M. C. Charlesworth, D. J. McCormick, and M. E. Bolander. 2003. Two-dimensional gel electrophoresis of synovial fluid: Method for detecting candidate protein markers for osteoarthritis. J. Orthopaed. Sci. 8: 482-490.   DOI   ScienceOn
17 Xiang, Y., T. Sekine, H. Nakamura, S. Imajoh-Ohmi, H. Fukuda, K. Nishioka, and T. Kato. 2004. Proteomic surveillance of autoimmunity in osteoarthritis: Identification of triosephosphate isomerase as an autoantigen in patients with osteoarthritis. Arthritis Rheumat. 50: 1511-1521.   DOI   ScienceOn