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http://dx.doi.org/10.4014/jmb.1110.10065

Genome Organization of Temperate Phage 11143 from Emetic Bacillus cereus NCTC11143  

Lee, Young-Duck (Department of Food and Biotechnology, College of Engineering, Gachon University)
Park, Jong-Hyun (Department of Food and Biotechnology, College of Engineering, Gachon University)
Publication Information
Journal of Microbiology and Biotechnology / v.22, no.5, 2012 , pp. 649-653 More about this Journal
Abstract
A temperate phage was isolated from emetic Bacillus cereus NCTC 11143 by mitomycin C and characterized by transmission electron microscopy and DNA and protein analyses. Whole genome sequencing of Bacillus phage 11143 was performed by GS-FLX. The phage has a dsDNA genome of 39,077 bp and a 35% G+C content. Bioinformatic analysis of the phage genome revealed 49 putative ORFs involved in replication, morphogenesis, DNA packaging, lysogeny, and host lysis. Bacillus phage 11143 could be classified as a member of the Siphoviridae family by morphology and genome structure. Genomic comparisons at the DNA and protein levels revealed homologous genetic modules with patterns and morphogenesis proteins similar to those of other Bacillus phages. Thus, Bacillus phages might have a mosaic genetic relationship.
Keywords
B. cereus; temperate phage; DNA sequence; sequence analysis;
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