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http://dx.doi.org/10.4014/jmb.1110.10055

Visualization of Candidate Division OP3 Cocci in Limonene-Degrading Methanogenic Cultures  

Rotaru, Amelia-Elena (Department of Microbiology, Max Planck Institute for Marine Microbiology)
Schauer, Regina (Department of Microbiology, Max Planck Institute for Marine Microbiology)
Probian, Christina (Department of Microbiology, Max Planck Institute for Marine Microbiology)
Mussmann, Marc (Department of Microbial Ecology, Max Planck Institute for Marine Microbiology)
Harder, Jens (Department of Microbiology, Max Planck Institute for Marine Microbiology)
Publication Information
Journal of Microbiology and Biotechnology / v.22, no.4, 2012 , pp. 457-461 More about this Journal
Abstract
Members of candidate division OP3 were detected in 16S rRNA gene clone libraries from methanogenic enrichment cultures that utilized limonene as a carbon and energy source. We developed probes for the visualization of OP3 cells. In situ hybridization experiments with newly designed OP3-specific probes [OP3-565 and Eub-338(VI)] revealed abundant small OP3 cocci attached to larger cells. Syntrophic Deltaproteobacteria, OP3 cells, and methanogens affiliating with Methanoculleus and Methanosaeta formed the limonene-degrading community.
Keywords
Candidate division OP3; monoterpene; limonene; hydrocarbon degradation; methanogenesis; syntrophy;
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