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http://dx.doi.org/10.4014/jmb.1108.08042

Identification of the Fur-Binding Site in Regulatory Region of the Vulnibactin-Receptor Gene in Vibrio vulnificus  

Lee, Hyun-Jung (Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health)
Lee, Kyu-Ho (Department of Life Science and Interdisciplinary Program of Integrated Biotechnology, Sogang University)
Publication Information
Journal of Microbiology and Biotechnology / v.22, no.1, 2012 , pp. 46-49 More about this Journal
Abstract
The Vibrio vulnificus vuuA gene, of which expression is repressed by a complex of iron and ferric uptake regulator (Fur), was characterized to localize the Fur-binding site in its upstream regulatory region. In silico analysis suggested the presence of two possible Fur-binding sites; one is a classical Fur-box and the other is a previously reported distinct Fur-binding site. Site-directed mutagenesis and DNase I protection assays revealed the binding site for the iron-Fur complex, which includes an extended inverted repeat containing a homologous sequence to the classical Fur-box.
Keywords
Fur; vulnibactin-receptor gene; Vibrio vulnificus;
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