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http://dx.doi.org/10.4014/jmb.1209.09058

Up-Regulation of $p27^{Kip1}$ Protects hES Cells from Differentiation-Associated and Caspase 3-Dependent Apoptosis  

Park, So-Hyun (Department of Pharmacy, College of Pharmacy, Hanyang University)
Kim, Min Kyoung (Drug Discovery Lab, Central Research Institute, ChoongWae Pharma Corp.)
Lee, Chul-Hoon (Department of Pharmacy, College of Pharmacy, Hanyang University)
Publication Information
Journal of Microbiology and Biotechnology / v.22, no.12, 2012 , pp. 1790-1794 More about this Journal
Abstract
Recently, it has been suggested that $p27^{Kip1}$, the cell cycle regulatory protein, plays a pivotal role in the progression of normal differentiation in murine embryonic stem (mES) cells. In the current study, we investigated the role of $p27^{Kip1}$ in the regulation of differentiation and apoptotic induction using Western blotting, quantitative real-time RT-PCR, and small interfering RNA (siRNA) assays and confocal laser scanning microscopic analysis of H9 human ES (hES) cells and H9-derived embryoid bodies (EBs) grown for 10 ($EB_{10}$) and 20 days ($EB_{20}$). Our results demonstrate that the proteins $p27^{Kip1}$ and cyclin D3 are strongly associated with cellular differentiation, and, for the first time, show that up-regulation of $p27^{Kip1}$ protects hES cells from inducing differentiation-associated and caspase 3-dependent apoptosis.
Keywords
$p27^{Kip1}$; cyclin D3; apoptosis; caspase 3; embryoid bodies; differentiation;
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