Browse > Article
http://dx.doi.org/10.4014/jmb.1207.07064

Cloning and Functional Verification of the Candida milleri HIS3 Gene Encoding Imidazoleglycerol Phosphate Dehydratase  

Park, Eun-Hee (Department of Food Science and Biotechnology, Kangwon National University)
Kwun, Se-Young (Department of Food Science and Biotechnology, Kangwon National University)
Han, Seung-Ah (Department of Food Science and Biotechnology, Kangwon National University)
Lee, Jong-Sub (Department of Food Science and Biotechnology, Kangwon National University)
Kim, Myoung-Dong (Department of Food Science and Biotechnology, Kangwon National University)
Publication Information
Journal of Microbiology and Biotechnology / v.22, no.10, 2012 , pp. 1441-1445 More about this Journal
Abstract
The entire nucleotide sequence of the HIS3 gene encoding imidazoleglycerol phosphate dehydratase (IGPD) in yeast Candida milleri CBS 8195 was determined. Sequence analysis revealed an open-reading frame of C. milleri HIS3 that spans 678 bp, encodes 226 amino acids, and shares 80.5% amino acid identity to Torulaspora delbrueckii IGPD, followed by that to Saccharomyces cerevisiae (79.6%). The cloned HIS3 gene complemented a his3 mutation in S. cerevisiae, suggesting that it encodes a functional IGPD in C. milleri CBS 8195. A new auxotrophic marker is now available for acid-tolerant yeast C. milleri.
Keywords
Candida milleri; imidazoleglycerol phosphate dehydratase; HIS3;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 0  (Related Records In Web of Science)
연도 인용수 순위
1 Pronk, J. T. 2002. Auxotrophic yeast strains in fundamental and applied research. Appl. Environ. Microbiol. 68: 2095-2100.   DOI   ScienceOn
2 Rosenbluh, A., M. Mevarech, Y. Koltin, and J. A. Gorman. 1985. Isolation of genes from Candida albicans by complementation in Saccharomyces cerevisiae. Mol. Gen. Genet. 200: 500-502.   DOI
3 Sambrook, J. and D. W. Russell. 2001. Molecular Cloning, pp. 1.119-1.122. 3rd Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
4 Struhl, K. and R. W. Davis. 1977. Production of a functional eukaryotic enzyme in Escherichia coli: Cloning and expression of the yeast structural gene for imidazole-glycerolphosphate dehydratase (his3). Proc. Natl. Acad. Sci. USA 74: 5255-5259.   DOI   ScienceOn
5 Sychrova, H., V. Braun, and J. L. Souciet. 2000. Sequence and organization analyses of a Zygosaccharomyces rouxii DNA fragment containing the HIS3 gene. Yeast 16: 581-587.   DOI   ScienceOn
6 Thireos, G., M. D. Penn, and H. Greer. 1984. 5' Untranslated sequences are required for the translational control of a yeast regulatory gene. Proc. Natl. Acad. Sci. USA 81: 5096-5100.   DOI   ScienceOn
7 Weinstock, K. G. and J. N. Strathern. 1993. Molecular genetics in Saccharomyces kluyveri: The HIS3 homolog and its use as a selectable marker gene in S. kluyveri and Saccharomyces cerevisiae. Yeast 9: 351-361.   DOI   ScienceOn
8 Yang, Z. 1997. PAML: A program package for phylogenetic analysis by maximum likelihood. Comput. Appl. Biosci. 13: 555-556.
9 You, T., I. Stansfield, M. C. Romano, A. J. P. Brown, and G. M. Coghill. 2011. Analysing GCN4 translational control in yeast by stochastic chemical kinetics modelling and simulation. BMC Syst. Biol. 5: 131.   DOI   ScienceOn
10 Baganz, F., A. Hayes, D. Marren, D. C. Gardner, and S. G. Oliver. 1997. Suitability of replacement markers for functional analysis studies in Saccharomyces cerevisiae. Yeast 13: 1563-1573.   DOI   ScienceOn
11 Basabe, L., I. Dominguez, and F. P. Chavez. 2004. Cloning and sequence analysis of the Candida utilis HIS3 gene. Biomolec.Eng. 21: 15-20.   DOI   ScienceOn
12 Boneau, C. A. 1960. The effects of violations of assumptions underlying the test. Psychol. Bull. 57: 49-64.
13 Di Segni, G., S. Gastaldi, M. Zamboni, and G. P. Tocchini-Valentini. 2011. Yeast pheromone receptor genes STE2 and STE3 are differently regulated at the transcription and polyadenylation level. Proc. Natl. Acad. Sci. USA 108: 17082-17086.   DOI   ScienceOn
14 Fink, G. R. 1964. Gene-enzyme relations in histidine biosynthesis in yeast. Science 146: 525-527.   DOI   ScienceOn
15 Gietz, R. D. and R. A. Woods. 2002. Transformation of yeast by lithium acetate/single-stranded carrier DNA/polyethylene glycol method. Methods Enzymol. 350: 87-96.
16 Glynn, S. E., P. J. Baker, S. E. Sedelnikova, C. L. Davies, T. C. Eadsforth, C. W. Levy, et al. 2005. Structure and mechanism of imidazoleglycerol-phosphate dehydratase. Structure 13: 1809-1817.   DOI   ScienceOn
17 Gullo, M., A. D. Romano, A. Pulvirenti, and P. Giudici. 2003. Candida humilis-dominant species in sourdoughs for the production of durum wheat bran flour bread. Int. J. Food Microbiol. 80: 55-59.   DOI   ScienceOn
18 Hope, I. A. and K. Struhl. 1986. Functional dissection of a eukaryotic transcriptional activator protein, GCN4 of yeast. Cell 46: 885-894.   DOI   ScienceOn
19 Hinnebusch, A. G. 1984. Evidence for translational regulation of the activator of general amino acid control in yeast. Proc. Natl. Acad. Sci. USA 81: 6442-6446.   DOI   ScienceOn
20 Hope, I. A. and K. Struhl. 1985. GCN4 protein, synthesized in vitro, binds HIS3 regulatory sequences: Implications for general control of amino acid biosynthetic genes in yeast. Cell 43:177-188.   DOI   ScienceOn
21 Jackson, A. P., J. A. Gamble, T. Yeomans, G. P. Moran, D. Saunders, D. Harris, et al. 2009. Comparative genomics of the fungal pathogens Candida dubliniensis and Candida albicans. Genome Res. 19: 2231-2244.   DOI   ScienceOn
22 Kitada, K., E. Yamaguchi, and M. Arisawa. 1995. Cloning of the Candida glabrata TRP1 and HIS3 genes, and construction of their disruptant strains by sequential integrative transformation. Gene 165: 203-206.   DOI   ScienceOn
23 Moslehi-Jenabian, S., L. L. Pedersen, and L. Jespersen. 2010. Beneficial effects of probiotic and food borne yeasts on human health. Nutrients 2: 449-473.   DOI
24 Mount, R. C., B. E. Jordan, and C. Hadfield. 1996. Transformation of lithium-treated yeast cells and the selection of auxotrophic and dominant markers. Methods Molec. Biol. 53: 139-145.
25 Park, E. H., D. H. Lee, J. H. Seo, and M. D. Kim. 2011. Cloning and characterization of a glyoxalase I gene from the osmotolerant yeast Candida magnoliae. J. Microbiol. Biotechnol. 21: 277-283.
26 Ames, B. N. 1957. The biosynthesis of histidine; D-erythroimidazoleglycerol phosphate dehydrase. J. Biol. Chem. 228:131-143.