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http://dx.doi.org/10.4014/jmb.1207.07031

Functional Mechanism of Plant Growth Retardation by Bacillus subtilis IJ-31 and Its Allelochemicals  

Kim, Won-Chan (Department of Agricultural Chemistry, College of Agriculture and Life Sciences, Kyungpook National University)
Rhee, In-Koo (Department of Agricultural Chemistry, College of Agriculture and Life Sciences, Kyungpook National University)
Publication Information
Journal of Microbiology and Biotechnology / v.22, no.10, 2012 , pp. 1375-1380 More about this Journal
Abstract
We previously isolated a rhizobacterium (Bacillus subtilis IJ-31) and demonstrated that its associated allelochemicals could indicate plant growth retardation. However, little is known about how the growth of plants is regulated by B. subtilis IJ-31 and its allelochemicals. In this study, we investigated whether plant growth retardation in this relationship occurred through the inhibition of gibberellin (GA) biosynthesis. GA $3{\beta}$-hydroxylase activity was found to be inhibited by B. subtilis IJ-31 and hydrocinnamic acid (HCA), which is one of the allelochemicals produced by B. subtilis IJ-31. Additionally, thin layer chromatography (TLC) demonstrated that B. subtilis IJ-31 culture broth and HCA both inhibit GA $3{\beta}$-hydroxylase (MBP-GA4) activity. The retardation of plants by HCA was then confirmed in vivo and in vitro using a Ryegrass and Arabidopsis growth retardation assay. Furthermore, treatment with either B. subtilis IJ-31 culture extract or its allelochemicals resulted in significant down-regulation of XTR9 gene expression in Arabidopsis. Overall, we identified the functional mechanism of plant growth retardation by B. subtilis IJ-31 and its allelochemicals.
Keywords
Bacillus subtilis; gibberellin $3{\beta}$-hydroxylase; allelochemicals; xyloglucan endotransglycosylases; hydrocinnamic acid;
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1 Smith, R. C. and S. C. Fry. 1991. Endotransglycosylation of xyloglucan in plant cell suspension cultures. Biochem. J. 279:529-535.
2 Strzelcyzk, E. and A. Pokojska-Burdziej. 1984. Production of auxin and gibberelin-like substance by mycorrhizal fungi, bacteria and actinomycetes isolated from soil and the mycorrhizosphere of pine (Pinus silverstris L.). Plant Soil 81: 185-194.   DOI   ScienceOn
3 Schippers, B., A. W. Bakker, and A. H. M. Bakker. 1987. Interactions of deleterious and beneficial rhizosphere microorganisms and the effect of cropping practices. Annu. Rev. Phytopathol. 25: 339-358.   DOI   ScienceOn
4 Smith, R. C., P. R. Matthews, P. H. D. Schünmnn, and P. M. Chandler. 1996. The regulation of leaf elongation and xyloglucan endotransglycosylase by gibberellin in 'Himalaya' barley (Hordeum vulgare L.). J. Exp. Bot. 47: 1395-1404.   DOI   ScienceOn
5 Wu, Y., W. G. Spollen, R. E. Sharp, P. R. Hetherington, and S. C. Fry. 1994. Root growth maintenance at low water potentials (increased activity of xyloglucan endotransglycosylase and its possible regulation by abscisic acid). Plant Physiol. 106: 607-615.
6 Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl. 1992. Short Protocols in Molecular Biology, 4th Ed. John Wiley & Sons, Inc., NY, USA.
7 Campbell, P. and J. Braam. 1999. In vitro activities of four xyloglucan endotransglycosylases from Arabidopsis. Plant J. 18: 371-382.   DOI   ScienceOn
8 Chen, F., H. Nonogaki, and K. J. Bardford. 2002. A gibberellinregulated xyloglucan endotransglycosylase gene is expressed in the endosperm cap during tomato seed germination. J. Exp. Bot. 53: 215-223.   DOI   ScienceOn
9 Darley, C. P., A. M. Forrester, and S. J. McQueen-Mason. 2001. The molecular basis of plant cell wall extension. Plant Mol. Biol. 47: 179-195.   DOI   ScienceOn
10 Feinberg, A. P. and B. Vogelstrin. 1983. A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132: 6-13.   DOI   ScienceOn
11 Gross, D. and B. Parthier. 1994. Novel natural substances acting in plant growth regulation. J. Plant Growth Regul. 13: 94-114.
12 Joo, G. J., Y. M. Kim, O. S. Lee, J. W. Kim, W. C. Kim, K. S. Song, et al. 2005. Optimization of culture condition for the hydrocinnamic acid production from Bacillus subtilis IJ-31. J. Korean Soc. Appl. Biol. Chem. 48: 207-211.
13 Lee, H. J., W. C. Kim, S. Y. Jeon, J. W. Kim, G. J. Joo, I. K. Rhee, and K. S. Song. 2003. Growth inhibitors of soybean seedling from Bacillus sp. IJ-31. Agric. Chem. Biotechnol. 46:100-104.
14 Nehl, D. B., S. J. Allen, and J. F. Brown. 1997. Deleterious rhizosphere bacteria: An integrating perspective. Appl. Soil Ecol. 5: 1-20.   DOI   ScienceOn
15 Lee, I. J., K. R. Foster, and P. W. Morgan. 1998. Photoperiod control of gibberellin levels and flowering in sorghum. Plant Physiol. 116: 1003-1010.   DOI   ScienceOn
16 MacMillan, J. 2002. Occurrence of gibberellins in vascular plants, fungi and bacteria. J. Plant Growth Regul. 20: 387-442.
17 MacMillan, J. and P. J. Suter. 1958. The occurrence of gibberellin A1 in higher plants: Isolation from the seed of runner bean (Phaseolus multiflorus). Naturwissenschaften 45: 46.
18 Nishitani, K. and R. Tominaga. 1992. Endo-xyloglucan transferase, a novel class of glycosyltransferase that catalyses transfer of a segment of xyloglucan molecules to another xyloglucan molecule. J. Biol. Chem. 257: 21058-21064.
19 Ortiz-Castro, R., H. A. Contreras-Cornejo, L. Macias-Rodriguez, and J. Lopez-Bucio. 2009. The role of microbial signals in plant growth and development.
20 Rademacher, W. 2000. Growth retardants: Effects on gibberellin biosynthesis and other metabolic pathways. Annu. Rev. Plant Physiol. Plant Mol. Biol. 51: 501-531.   DOI   ScienceOn
21 Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA.