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http://dx.doi.org/10.4014/jmb.1203.02056

Biocontrol of Anthracnose in Pepper Using Chitinase, ${\beta}$-1,3 Glucanase, and 2-Furancarboxaldehyde Produced by Streptomyces cavourensis SY224  

Lee, So Youn (Department of Agricultural Chemistry, Chonnam National University)
Tindwa, Hamisi (Department of Agricultural Chemistry, Chonnam National University)
Lee, Yong Seong (Department of Agricultural Chemistry, Chonnam National University)
Naing, Kyaw Wai (Department of Agricultural Chemistry, Chonnam National University)
Hong, Seong Hyun (Department of Agricultural Chemistry, Chonnam National University)
Nam, Yi (Ansung Training Institute, National Agricultural Cooperative Federation)
Kim, Kil Yong (Department of Agricultural Chemistry, Chonnam National University)
Publication Information
Journal of Microbiology and Biotechnology / v.22, no.10, 2012 , pp. 1359-1366 More about this Journal
Abstract
A strain of Streptomyces cavourensis subsp. cavourensis (coded as SY224) antagonistic to Colletotrichum gloeosporioides infecting pepper plants was isolated. SY224 produced lytic enzymes such as chitinase, ${\beta}$-1,3-glucanase, lipase, and protease in respective assays. To examine for antifungal activity, the treatments amended with the nonsterilized supernatant resulted in the highest growth inhibition rate of about 92.9% and 87.4% at concentrations of 30% and 10%, respectively. However, the sterilized treatments (autoclaved or chloroform treated) gave a lowered but significant inhibitory effect of about 63.4% and 62.6% for the 10% supernatant concentration, and 75.2% and 74.8% for the of 30% supernatant concentration in the PDA agar medium, respectively, indicative of the role of a non-protein, heat stable compound on the overall effect. This antifungal compound, which inhibited spore germination and altered hyphal morphology, was extracted by EtOAc and purified by ODS, silica gel, Sephadex LH-20 column, and HPLC, where an active fraction was confirmed to be 2-furancarboxaldehyde by GS-CI MS techniques. These results suggested that SY224 had a high potential in the biocontrol of anthracnose in pepper, mainly due to a combined effect of lytic enzymes and a non-protein, heat-stable antifungal compound, 2-furancarboxaldehyde.
Keywords
Antifungal activity; ${\beta}$-1,3 glucanase; chitinase; 2-furancarboxaldehyde Streptomyces cavourensis;
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