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http://dx.doi.org/10.4014/jmb.1010.10061

Cloning and Expression of a bpr Gene Encoding Bacillopeptidase F from Bacillus amyloliquefaciens CH86-1  

Kwon, Gun-Hee (Division of Applied Life Science (BK21 Program), Graduate School, Gyeongsang National University)
Park, Jae-Yong (Department of Food Science and Nutrition, Catholic University of Daegu)
Kim, Jong-Sang (School of Applied Biosciences, Kyungpook National University)
Lim, Jin-Kyu (School of Applied Biosciences, Kyungpook National University)
Park, Cheon-Seok (Department of Food Science and Biotechnology, Kyung Hee University)
Kwon, Dae-Young (Food Function Research Division, Korea Food Research Institute)
Kim, Jeong-Hwan (Division of Applied Life Science (BK21 Program), Graduate School, Gyeongsang National University)
Publication Information
Journal of Microbiology and Biotechnology / v.21, no.5, 2011 , pp. 515-518 More about this Journal
Abstract
A gene encoding bacillopeptidase F, bpr86-1, was cloned from B. amyloliquefaciens CH86-1 isolated from cheonggukjang. This gene could encode a preproenzyme of 1,431 amino acids. When bpr86-1 was introduced into B. subtilis WB600 via pHY300PLK, an E. coli-Bacillus shuttle vector, the transformant showed fibrinolytic activity. During growth on LB, the fibrinolytic activity of cells increased sharply when they entered the stationary phase. The highest activity (761.4 mU/mg protein) was observed at 96 h of cultivation.
Keywords
Bacillus amyloliquefaciens CH86-1; bacillopeptidase F; bpr gene; fibrinolytic enzymes;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
Times Cited By Web Of Science : 0  (Related Records In Web of Science)
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