Cloning and Expression of a bpr Gene Encoding Bacillopeptidase F from Bacillus amyloliquefaciens CH86-1
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Kwon, Gun-Hee
(Division of Applied Life Science (BK21 Program), Graduate School, Gyeongsang National University)
Park, Jae-Yong (Department of Food Science and Nutrition, Catholic University of Daegu) Kim, Jong-Sang (School of Applied Biosciences, Kyungpook National University) Lim, Jin-Kyu (School of Applied Biosciences, Kyungpook National University) Park, Cheon-Seok (Department of Food Science and Biotechnology, Kyung Hee University) Kwon, Dae-Young (Food Function Research Division, Korea Food Research Institute) Kim, Jeong-Hwan (Division of Applied Life Science (BK21 Program), Graduate School, Gyeongsang National University) |
1 | Bradford, M. M. 1976. Rapid and sensitive methods for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254. DOI ScienceOn |
2 | Choi, N.-S., D.-M. Chung, C. H. Ryu, K.-S. Yoon, P. J. Maeng, and S. H. Kim. 2006. Identification of three extracellular proteases from Bacillus subtilis KCTC 3014. J. Microbiol. Biotechnol. 16: 457-464. |
3 | Jeong, S.-J., G.-H. Kwon, J. Chun, J. S. Kim, C.-S. Park, D. Y. Kwon, and J. H. Kim. 2007. Cloning of fibrinolytic enzyme gene from Bacillus subtilis isolated from cheonggukjang and its expression in protease-deficient Bacillus subtilis strains. J. Microbiol. Biotechnol. 17: 1018-1023. |
4 | Lee, A. R., G. M. Kim, J.-Y. Park, H. D. Jo, J. Cha, Y.-S. Song, J. Chun, and J. H. Kim. 2010. Characterization of a 27 kDa fibrinolytic enzyme from Bacillus amyloliquefaciens CH86-1 isolated from cheonggukjang. J. Kor. Soc. Appl. Biol. Chem. 53: 56-61. DOI |
5 | Kim, G. M., A. R. Lee, K. W. Lee, J.-Y. Park, J. Chun, J. Cha, Y.-S. Song, and J. H. Kim. 2009. Characterization of a 27 kDa fibrinolytic enzyme from Bacillus amyloliquefaciens CH51 isolated from cheonggukjang. J. Microbiol. Biotechnol. 19: 997-1004. DOI |
6 | Kim, S.-H., N.-S. Choi, and W. Y. Lee. 1998. Fibrin zymography: A direct analysis of fibrinolytic enzymes on gels. Anal. Biochem. 263: 115-116. DOI ScienceOn |
7 | Lee, A. R., G. M. Kim, G.-H. Kwon, K. W. Lee, J.-Y. Park, J. Chun, J. Cha, Y.-S. Song, and J. H. Kim. 2010. Cloning of aprE86-1 gene encoding a 27 kDa mature fibrinolytic enzyme from Bacillus amyloliquefaciens CH86-1. J. Microbiol. Biotechnol. 20: 370-374. |
8 | Veening, J.-W., O. A. Igoshin, R. T. Eijlander, R. Nijland, L. W. Hamoen, and O. Kuipers. 2008. Transient heterogeneity in extracellular protease production by Bacillus subtilis. Mol. Syst. Biol. 184: 1-15. |
9 | Park, C. H., S. J. Lee, S. G. Lee, W. S. Lee, and S. M. Byun. 2004. Hetero- and autoprocessing of the extracellular metalloprotease (Mpr) in Bacillus subtilis. J. Bacteriol. 186: 6457-6464. DOI ScienceOn |
10 | Peng, Y., X. Yang, and Y. Zhang. 2005. Microbial fibrinolytic enzymes: An overview of source, production, properties, and thrombolytic activity in vivo. Appl. Microbiol. Biotechnol. 69: 126-132. DOI ScienceOn |
11 | Wu, X. C., S. Nathoo, A. S.-H. Pang, T. Carne, and S. L. Wong. 1990. Cloning, genetic organization, and characterization of a structural gene encoding bacillopeptidase F from Bacillus subtilis. J. Biol. Chem. 265: 6845-6850. |
12 | Wu, X. C., W. Lee, L. Tran, and S. L. Wong. 1991. Engineering a Bacillus subtilis expression-secretion system with a strain deficient in six extracellular proteases. J. Bacteriol. 173: 4952- 4958. |
13 | Yamagata, Y., R. Abe, Y. Fujita, and E. Ichishima. 1995. Molecular cloning and nucleotide sequence of the 90k serine protease gene, hspK, from Bacillus subtilis (natto) No. 16. Curr. Microbiol. 31: 340-344. DOI ScienceOn |
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