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http://dx.doi.org/10.4014/jmb.1012.12013

Evaluation of the Potential Risk of Porcine Endogenous Retrovirus (PERV) Infection in Nude Mice  

Bae, Eun-Hye (Department of Microbiology and Institute of Basic Science, Dankook University)
Jung, Yong-Tae (Department of Microbiology and Institute of Basic Science, Dankook University)
Publication Information
Journal of Microbiology and Biotechnology / v.21, no.4, 2011 , pp. 387-390 More about this Journal
Abstract
Nude mice (BALB/c) were grafted with human 293 cells and PERV (porcine endogenous retrovirus)-IRES-EGFP (a packageable retroviral vector plasmid containing an internal ribosome entry site-enhanced green fluorescent protein)-producing pig PK15 cells in order to determine whether the pig cells could transmit PERV-IRES-EGFP to mice and human 293 cells in vivo. None of the transplanted human 293 cell lines were infected by PERV, but PCR analysis identified PERV-B provirus integration into both the heart and salivary gland of the inoculated nude mice. Our data indicate that hearts and salivary glands can be used to identify PERV-B receptors.
Keywords
Animal model; nude mice; porcine endogenous retroviruses; replication-defective retroviral vector; xenotransplantation;
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