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http://dx.doi.org/10.4014/jmb.1008.08031

A RAPD-PCR Method for the Rapid Detection of Bacillus cereus  

Lee, Ji-Yeon (Division of Applied Life Science (BK21), Graduate School, Gyeongsang National University)
Kwon, Gun-Hee (Institute of Agriculture and Life Science, Gyeongsang National University)
Park, Jae-Yong (Department of Food Science and Nutrition, Catholic University of Daegu)
Park, Cheon-Seok (Department of Food Science and Technology, Kyunghee University)
Kwon, Dae-Young (Food Function Research Division, Korea Food Research Institute)
Lim, Jin-Kyu (Shool of Applied Bioscience, Kyungpook National University)
Kim, Jong-Sang (Shool of Applied Bioscience, Kyungpook National University)
Kim, Jeong-Hwan (Division of Applied Life Science (BK21), Graduate School, Gyeongsang National University)
Publication Information
Journal of Microbiology and Biotechnology / v.21, no.3, 2011 , pp. 274-276 More about this Journal
Abstract
Distinction of Bacillus cereus from other closely related bacilli is challenging and new efficient methods are continually demanded. From our previous work on RAPD profiles of bacilli, we found a possibility that B. cereus strains could be distinguished from other bacilli. In this work, RAPD-PCR profiles of B. cereus strains were obtained using a 10-mer (S30) as a primer, and a B. cereus specific 0.91-kb band was produced from all tested strains. The RAPD-PCR procedure also successfully detected B. cereus from spiked cheonggukjang when B. cereus cells were present at more than $10^2$/g sample.
Keywords
Bacillus cereus; food poisoning; rapid detection; RAPD-PCR;
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