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http://dx.doi.org/10.4014/jmb.1001.01017

Role of Exopolymeric Substances (EPS) in the Stability of the Biofilm of Thiomonas arsenivorans Grown on a Porous Mineral Support  

Michel, Caroline (BRGM, EPI/ECO)
Garrido, Francis (BRGM, EPI/ECO)
Roche, Emilie (BRGM, EPI/ECO)
Belval, Sylvain Challan (BRGM, EPI/ECO)
Dictor, Marie-Christine (BRGM, EPI/ECO)
Publication Information
Journal of Microbiology and Biotechnology / v.21, no.2, 2011 , pp. 183-186 More about this Journal
Abstract
Biochemical methods were selected to evaluate the role of exopolymeric substances in the stability of biofilms used in bioremediation processes. Biofilms of Thiomonas arsenivorans formed on pozzolana were thus treated with pronase (protein target), lectins (Con A or PNA), calcofluor or periodic acid (polysaccharides target), DNase (DNA target), and lipase (triglycerides target). Neither protease nor DNase treatments had any effect on bacterial adhesion. Lectins and calcofluor treatments mainly affected young biofilms. Lipase treatment had a noticeable effect on biofilm stability whatever the biofilm age. Results suggest that it would be an increased resistance of mature biofilms that protects them from external attacks.
Keywords
Exopolymeric substances (EPS); pozzolana; biofilm stability; enzymatic and chemical treatments; Thiomonas arsenivorans;
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