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http://dx.doi.org/10.4014/jmb.1007.07027

Phenotypic and Genotypic Characterization of Salmonella spp. Isolated from Pigs and their Farm Environment in Korea  

Lim, Suk-Kyung (National Veterinary Research and Quarantine Service)
Byun, Jung-Ryul (National Veterinary Research and Quarantine Service)
Nam, Hyang-Mi (National Veterinary Research and Quarantine Service)
Lee, Hee-Soo (National Veterinary Research and Quarantine Service)
Jung, Suk-Chan (National Veterinary Research and Quarantine Service)
Publication Information
Journal of Microbiology and Biotechnology / v.21, no.1, 2011 , pp. 50-54 More about this Journal
Abstract
This study's objective was to determine the prevalence of Salmonella spp. in pigs and their farm environments in Korea, and to investigate the relationship between the strains based on their phenotypic and genotypic characteristics. A total of 36 Salmonella spp. were isolated in this study: 18 isolates from 492 pigs (3.7%) and 18 isolates from 418 (4.3%) farmhouse environmental samples from 16 different pig farms. Of the Salmonella strains isolated from the numerous environmental samples, the highest prevalence was observed in slurry or manure, followed by partitions, farmer's hands, floors, water/nipples, ventilation sources, and feed, respectively. All the Salmonella isolates originating from different farms were genetically distinct. In three farms, however, identical phage types and pulse-field gel electrophoresis patterns were observed among Salmonella isolates from pig feces and environmental samples. This study suggests that environments contaminated with Salmonella could pose an infection risk to pigs on pig farms.
Keywords
Salmonella spp.; pig; environment; Korea;
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1 Jensen, A. N., S. Dalsqaard, A. Stockmarr, E. M. Nielsen, and D. L. Baggesen. 2006. Survival and transmission of Salmonella enterica serovar Typhimurium in an outdoor organic pig farming environment. Appl. Environ. Microbiol. 72: 1833-1842.   DOI   ScienceOn
2 Lee, W. C., M. J. Lee, J. S. Kim, and S. Y. Park. 2001. Foodborne illness outbreaks in Korea and Japan studied retrospectively. J. Food Prot. 64: 899-902.   DOI
3 Lim, S. K., H. S. Lee, H. M. Nam, S. C. Jung, H. B. Koh, and I. S. Roh. 2009. Antimicrobial resistance and phage types of Salmonella isolates from healthy and diarrheic pigs in Korea. Foodborne Pathog. Dis. 6: 981-987.   DOI   ScienceOn
4 Mannion, C., P. B. Lynch, J. Egan, and F. C. Leonard. 2007. Seasonal effects on the survival characteristics of Salmonella Typhimurium and Salmonella Derby in pig slurry during storage. J. Appl. Microbiol. 103: 1386-1392.   DOI   ScienceOn
5 Merialdi, G., G. Barigazzi, P. Bonilauri, C. Tittarelli, M. Bonci, M. D'incau, and M. Dottori. 2008. Longitudinal study of Salmonella infection in Italian farrow-to-finish swine herds. Zoonoses Public Health, 55: 222-226.   DOI   ScienceOn
6 Sandvand, D., L. B. Jensen, D. L. Baggesen, and S. B. Baloda. 2000. Persistence of a Salmonella enterica serotype Typhimurium clone in Danish pig production units and farmhouse environment studied by pulsed field gel electrophoresis (PFGE). FEMS Microbiol. Lett. 187: 21-25.   DOI   ScienceOn
7 Barber, D. A., P. B. Bahnson, R. Isaacson, C. J. Jones, and R. M. Weigel. 2002. Distribution of Salmonella in swine production ecosystems. J. Food Prot. 65: 1861-1868.   DOI
8 Anderson, E. S., L. R. Ward, M. J. Saxe, and J. D. de Sa. 1977. Bacteriophage-typing designations of Salmonella Typhimurium. J. Hyg. Lond. 78: 297-330.   DOI
9 Baloda, S. B., L. Christensen, and S. Trajcevska. 2001. Persistence of a Salmonella enterica serovar Typhimurium DT12 clone in a piggery and in agricultural soil amended with Salmonella-contaminated slurry. Appl. Environ. Microbiol. 67: 2859-2862.   DOI   ScienceOn
10 Bauer, A. W., M. M. Kirby, J. C. Sherris, and M. Turck. 1996. Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol. 45: 493-496.
11 Boyen, F., F. Haesebrouck, D. Maes, F. Van Immerseel, R. Ducatelle, and F. Pasmans. 2008. Non-typhoidal Salmonella infections in pigs: A closer look at epidemiology, pathogenesis and control. Vet. Microbiol. 130: 1-19.   DOI   ScienceOn
12 Cheong, H. J., Y. J. Lee, L. S. Hwang, S. Y. Kee, H. W. Cheong, J. Y. Song, et al. 2007. Characteristics of non-typhoidal Salmonella isolates from human and broiler-chickens in southwestern Seoul, Korea. J. Korean Med. Sci. 22: 773-778.   DOI   ScienceOn
13 Clinical Laboratory Standards Institute. 2007. Performance Standards for Antimicrobial Susceptibility Testing: 13th Informational supplement. M100-S17. CLSI, Wayne, PA.
14 Suh, D. K. and S. C. Jung. 2005. Epidemiological characteristics of Salmonella spp. isolated from different stages of commercial swine farms. Korean J. Vet. Res. 45: 179-183.
15 Ribor, E. M., M. A. Fair, R. Gautom, D. N. Cameron, S. B. Hunter, B. Swaminathan, and T. J. Barrett. 2006. Standardization of pulsed-field gel electrophoresis protocols for the subtyping of Escherichia coli O157:H7, Salmonella, and Shigella for PulseNet. Foodborne Pathog. Dis. 3: 59-67.   DOI   ScienceOn
16 Rosengren, L. B., C. L. Waldner, R. J. Reid-Smith, M. E. McFall, and A. Rajic. 2008. Antimicrobial resistance of fecal Salmonella spp. isolated from all phases of pig production in 20 herds in Alberta and Saskatchewan. Can. J. Vet. Res. 72: 151-159.
17 Popoff, M. Y. and I. le Minor. 1992. Antigenic Formulas of the Salmonella Servars. WHO Collaborating Center for Reference and Research on Salmonella. Institute Pasteur, Paris.
18 Poppe, C., L. Martin, A. Muckle, M. Archambault, S. McEwen, and E. Weir. 2006. Characterization of antimicrobial resistance of Salmonella Newport isolated from animals, the environment, and animal food products in Canada. Can. J. Vet. Res. 70: 105-114.
19 Fedorka-Cray, P. J., S. C. Whipp, R. E. Isaacson, N. Nord, and K. Lager. 1994. Transmission of Salmonella Typhimurium to swine. Vet. Microbiol. 41: 333-344.   DOI   ScienceOn
20 Jensen, A. N., G. Sorensen, D. L. Boggesen, R. Bodker, and J. Hoorfar. 2003. Addition of novobiocin in pre-enrichment step can improve Salmonella culture protocol of modified semisolid Rappaport-Vassiliadis. J. Microbiol. Methods 55: 249-255.   DOI   ScienceOn
21 Wasyl, D., D. Sandvand, M. N. Skov, and D. L. Baggesen. 2006. Epidemiological characteristics of Salmonella Typhimurium isolated from animals and feed in Poland. Epidemiol. Infect. 134: 179-185.
22 Williams, J. E. and S. T. Benson. 1978. Survival of Salmonella Typhimurium in poultry feed and litter at three temperatures. Avian Dis. 22: 742-747.   DOI   ScienceOn