[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.4014/jmb.1006.06044

Bioconversion of Acrylonitrile to Acrylic Acid by Rhodococcus ruber Strain AKSH-84

Kamal, Ahmed (Chemical Biology Laboratory, Indian Institute of Chemical Technology)
Kumar, M. Shiva (Chemical Biology Laboratory, Indian Institute of Chemical Technology)
Kumar, C. Ganesh (Chemical Biology Laboratory, Indian Institute of Chemical Technology)
Shaik, Thokhir Basha (Chemical Biology Laboratory, Indian Institute of Chemical Technology)

Publication Information

Journal of Microbiology and Biotechnology / v.21, no.1, 2011 , pp. 37-42 More about this Journal

Abstract

A new versatile acrylonitrile-bioconverting strain isolated from a petroleum-contaminated sludge sample and identified as Rhodococcus ruber AKSH-84 was used for optimization of medium and biotransformation conditions for nitrilase activity to produce acrylic acid. A simple and rapid HPLC protocol was optimized for quantification of acrylic acid, acrylamide, and acrylonitrile. The optimal medium conditions for nitrilase activity were pH of 7.0, temperature of $30^{\circ}C$ , agitation of 150 rpm, and inoculum level of 2%. Glycerol as a carbon source and sodium nitrate as the nitrogen source provided good nutritional sources for achieving good biotransformation. Nitrilase activity was constitutive in nature and was in the exponential growth phase after 24 h of incubation under optimal conditions without addition of any inducer. The substrate preference was acrylonitrile and acetonitrile. The present work demonstrates the biotransformation of acrylonitrile to acrylic acid with the new strain, R. ruber AKSH-84, which can be used in green biosynthesis of acrylic acid for biotechnological processes. The nitrilase produced by the isolate was purified and characterized.

Keywords

Rhodococcus ruber; acrylic acid; acrylonitrile; nitrilase; biotransformation;

Citations & Related Records

Times Cited By Web Of Science : 1 (Related Records In Web of Science)

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