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http://dx.doi.org/10.4014/jmb.1101.01018

Cloning of metK from Actinoplanes teichomyceticus ATCC31121 and Effect of Its High Expression on Antibiotic Production  

Kim, Du-Yeong (Department of Food Science and Biotechnology, Kyungnam University)
Hwang, Yong-Il (Department of Food Science and Biotechnology, Kyungnam University)
Choi, Sun-Uk (Department of Food Science and Biotechnology, Kyungnam University)
Publication Information
Journal of Microbiology and Biotechnology / v.21, no.12, 2011 , pp. 1294-1298 More about this Journal
Abstract
A metK gene encoding S-adenosyl-L-methionine synthetase was cloned from the non-Streptomyces actinomycetes, Actinoplanes teichomyceticus ATCC31121. In order to evaluate the effect of the metK expression on antibiotic production in actinomycetes, an expression vector harboring the metK gene was constructed and introduced into Streptomyces lividans TK24 and A. teichomyceticus, and the antibiotic production of the exconjugants was assessed. As a result, it was determined that the expression of metK induced 17-fold and 2.2-fold increases in actinorhodin production from S. lividans TK24 and teicoplanin production from A. teichomyceticus, respectively, compared with the control strains.
Keywords
Actinoplanes teichomyceticus; teicoplanin; metK; cloning; actinorhodin;
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1 Bauernfeind, A. and C. Petermuller. 1982. In vitro activity of teichomycin A2 in comparison with penicillin and vancomycin against Gram-positive cocci. Eur. J. Clin. Microbiol. 1: 278-281.   DOI   ScienceOn
2 Bibb, M. J., P. R. Findlay, and M. W. Johnson. 1984. The relationship between base composition and codon usage in bacterial genes and its use for the simple and reliable identification of protein-coding sequences. Gene 30: 157-166.   DOI
3 Bibb, M. J., G. R. Janssen, and J. Ward. 1985. Cloning and analysis of the promoter region of the erythromycin resistance gene (ermE) of Streptomyces erythraeus. Gene 38: 215-226.   DOI   ScienceOn
4 Bierman, M., R. Logan, K. O'Brien, E. T. Seno, R. N. Rao, and B. E. Schoner. 1992. Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp. Gene 116: 43-49.   DOI   ScienceOn
5 Borghi, A., P. Antonini, M. Zanol, P. Ferrari, L. F. Zerilli, and G. C. Lancini. 1989. Isolation and structure determination of two new analogs of teicoplanin, a glycopeptide antibiotic. J. Antibiot. (Tokyo) 42: 361-366.   DOI
6 Chiang, P. K., R. K. Gordon, J. Tal, G. C. Zeng, B. P. Doctor, K. Pardhasaradhi, and P. P. McCann. 1996. S-Adenosylmethionine and methylation. FASEB J. 10: 471-480.
7 Ha, H. S., Y. I. Hwang, and S. U. Choi. 2008. Application of conjugation using phiC31 att/int system for Actinoplanes teichomyceticus, a producer of teicoplanin. Biotechnol. Lett. 30: 1233-1238.   DOI   ScienceOn
8 Heydorn, A., T. Suhr-Jessen, and J. Nielsen. 1999. Growth and production kinetics of a teicoplanin producing strain of Actinoplanes teichomyceticus. J. Antibiot. (Tokyo) 52: 40-44.   DOI   ScienceOn
9 Kieser, T., M. J. Bibb, K. F. Chater, and D. A. Hopwood. 2000. Practical Streptomyces Genetics. The John Innes Foundation, Norwich, UK.
10 Kim, D. J., J. H. Huh, Y. Y. Yang, C. M. Kang, I. H. Lee, C. G. Hyun, et al. 2003. Accumulation of S-adenosyl-L-methionine enhances production of actinorhodin but inhibits sporulation in Streptomyces lividans TK23. J. Bacteriol. 185: 592-600.   DOI   ScienceOn
11 Lu, S. C. 2000. S-Adenosylmethionine. Int. J. Biochem. Cell. Biol. 32: 391-395.   DOI   ScienceOn
12 Motamedi, H., A. Shafiee, and S. J. Cai. 1995. Integrative vectors for heterologous gene expression in Streptomyces spp. Gene 160: 25-31.   DOI   ScienceOn
13 Oh, T. J., N. P. Niraula, K. Liou, and J. K. Sohng. 2010. Identification of the duplicated genes for S-adenosyl-L-methionine synthetase (metK1-sp and metK2-sp) in Streptomyces peucetius var. caesius ATCC 27952. J. Appl. Microbiol. 109: 398-407.
14 Okamoto, S., A. Lezhava, T. Hosaka, Y. Okamoto-Hosoya, and K. Ochi. 2003. Enhanced expression of S-adenosylmethionine synthetase causes overproduction of actinorhodin in Streptomyces coelicolor A3(2). J. Bacteriol. 185: 601-609.   DOI   ScienceOn
15 Parenti, F., G. Beretta, M. Berti, and V. Arioli. 1978. Teichomycins, new antibiotics from Actinoplanes teichomyceticus nov. sp. I. Description of the producer strain, fermentation studies and biological properties. J. Antibiot. (Tokyo) 31: 276-283.   DOI
16 Rao, R. N., N. A. Richardson, and S. Kuhstoss. 1987. Cosmid shuttle vectors for cloning and analysis of Streptomyces DNA. Methods Enzymol. 153: 166-198.
17 Sambrook, J. D. and W. Russell. 2001. Molecular Cloning: A Laboratory Manual, 3rd Ed. Cold Spring Harbor, New York. USA.
18 Smokvina, T., P. Mazodier, F. Boccard, C. J. Thompson, and M. Guerineau. 1990. Construction of a series of pSAM2-based integrative vectors for use in actinomycetes. Gene 94: 53-59.   DOI   ScienceOn
19 Zhang, X., M. Fen, X. Shi, L. Bai, and P. Zhou. 2008. Overexpression of yeast S-adenosylmethionine synthetase metK in Streptomyces actuosus leads to increased production of nosiheptide. Appl. Microbiol. Biotechnol. 78: 991-995.   DOI   ScienceOn
20 Zhao, X. Q., B. Gust, and L. Heide. 2010. S-Adenosylmethionine (SAM) and antibiotic biosynthesis: Effect of external addition of SAM and of overexpression of SAM biosynthesis genes on novobiocin production in Streptomyces. Arch. Microbiol. 192: 289-297.   DOI   ScienceOn