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http://dx.doi.org/10.4014/jmb.1102.02019

Monitoring of Leuconostoc Population During Sauerkraut Fermentation by Quantitative Real-Time Polymerase Chain Reaction  

Kim, So-Young (Department of Agrofood Resources, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA))
Yoo, Ki-Seon (Department of Food Science and Technology, Research Center for Bioresource and Health, Chungbuk National University)
Kim, Yu-Jin (Department of Food Science and Technology, Research Center for Bioresource and Health, Chungbuk National University)
Seo, Eun-Young (Department of Food Science and Technology, Research Center for Bioresource and Health, Chungbuk National University)
Kim, Beom-Soo (Department of Chemical Engineering, Chungbuk National University)
Han, Nam-Soo (Department of Food Science and Technology, Research Center for Bioresource and Health, Chungbuk National University)
Publication Information
Journal of Microbiology and Biotechnology / v.21, no.10, 2011 , pp. 1069-1072 More about this Journal
Abstract
A real-time PCR assay method was established to monitor Leuconostoc spp. populations via specific amplification of the dextransucrase gene. Quantification of L. mesenteroides B-512F using both genomic DNA and cell suspensions yielded a log-linear correlation spanning approximately 5 log units. By using this method, monitoring changes of Leuconostoc spp. during sauerkraut fermentation was successfully accomplished with accuracy after inoculation of starter and sugars (sucrose and maltose).
Keywords
Sauerkraut; Leuconostoc; real-time PCR; monitoring; dextransucrase;
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Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 0  (Related Records In Web of Science)
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