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http://dx.doi.org/10.4014/jmb.1103.03040

Isolation and Identification of Antitumor Promoters from the Seeds of Cassia tora  

Park, Yeung-Beom (Department of Food Processing and Bakery, Gangwon Provincial College)
Kim, Seon-Bong (Department of Food Science and Technology/Institute of Food Science, Pukyong National University)
Publication Information
Journal of Microbiology and Biotechnology / v.21, no.10, 2011 , pp. 1043-1048 More about this Journal
Abstract
A methanol extract of Cassia tora seeds was successively partitioned with diethyl ether, chloroform, ethyl acetate, and water, and the antitumor-promoting activity of the solvent fractions was determined by inhibition of Epstein-Barr virus early antigen (EBV-EA) activation induced by teleocidin B-4 in Raji cells. The diethyl ether (68.7%) and chloroform (91.2%) fractions and the hydrolysate (94.3%) of the ethyl acetate fraction had strong inhibitory activities. The chloroform and ethyl acetate fractions were chromatographed on silica gel and further purified by HPLC. Three active compounds, obtusifolin-2-glucoside (75.0%), chryso-obtusin-6-glucoside (56.8%), and norrubrofusarin-6-glucoside (39.4%), were obtained from the ethyl acetate fraction, and two active compounds, questin (97.9%) and chryso-obtusin (53.8%), were isolated from the chloroform fraction.
Keywords
Epstein-Barr virus; antitumor promoter; Cassia tora; anthraquinone;
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