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http://dx.doi.org/10.4014/jmb.1105.05020

Chemical Modification of Botryosphaeran: Structural Characterization and Anticoagulant Activity of a Water-Soluble Sulfonated ($1{\rightarrow}3$)($1{\rightarrow}6$)-${\beta}$-D-Glucan  

Brandi, Jamile (Departamento de Fisica, Quimica e Biologia, Faculdade de Ciencias e Technologia, Universidade Estadual Paulista)
Oliveira, Eder C. (Departamento de Fisica, Quimica e Biologia, Faculdade de Ciencias e Technologia, Universidade Estadual Paulista)
Monteiro, Nilson K. (Departamento de Fisica, Quimica e Biologia, Faculdade de Ciencias e Technologia, Universidade Estadual Paulista)
Vasconcelos, Ana Flora D. (Departamento de Fisica, Quimica e Biologia, Faculdade de Ciencias e Technologia, Universidade Estadual Paulista)
Dekker, Robert F.H. (Biorefining Research Institute, Lakehead University)
Barbosa, Aneli M. (Departamento de Bioquimica e Biotechnologia, Universidade Estadual de Londrina)
Silveira, Joana L.M. (Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Parana)
Mourao, Paulo A.S. (Laboratorio de Tecido Conjuntivo, H. U. Clementino Fraga Filho, Universidade Federal do Rio de Janeiro)
Silva, Maria De Lourdes Corradi Da (Departamento de Fisica, Quimica e Biologia, Faculdade de Ciencias e Technologia, Universidade Estadual Paulista)
Publication Information
Journal of Microbiology and Biotechnology / v.21, no.10, 2011 , pp. 1036-1042 More about this Journal
Abstract
The exopolysaccharide botryosphaeran ($EPS_{GLC}$; a ($1{\rightarrow}3$)($1{\rightarrow}6$)-${\beta}$-D-glucan from Botryosphaeria rhodina MAMB-05) was sulfonated to produce a water-soluble fraction ($EPS_{GLC}$-S) using pyridine and chlorosulfonic acid in formamid. This procedure was then repeated twice to produce another fraction ($EPS_{GLC}$-RS) with a higher degree of substitution (DS, 1.64). The purity of each botryosphaeran sample (unsulfonated and sulfonated) was assessed by gel filtration chromatography (Sepharose CL-4B), where each polysaccharide was eluted as a single symmetrical peak. The structures of the sulfonated and re-sulfonated botryosphaerans were investigated using ultraviolet-visible (UV-Vis), Fourier-transform infrared (FT-IR), and $^{13}C$ nuclear magnetic resonance ($^{13}C$ NMR) spectroscopies. $EPS_{GLC}$ and $EPS_{GLC}$-RS were also assayed for anticoagulation activity, and $EPS_{GLC}$-RS was identified as an anticoagulant.
Keywords
Botryosphaeran; sulfonation; anticoagulant activity; Botryosphaeria rhodina MAMB-05;
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