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http://dx.doi.org/10.4014/jmb.1001.02023

A Monoclonal Antibody That Specifically Binds Chitosan In Vitro and In Situ on Fungal Cell Walls  

Schubert, Max (Fraunhofer Institute for Molecular Biology and Applied Ecology (IME))
Agdour, Siham (Fraunhofer Institute for Molecular Biology and Applied Ecology (IME))
Fischer, Rainer (Fraunhofer Institute for Molecular Biology and Applied Ecology (IME))
Olbrich, Yvonne (RWTH Aachen University, Institute for Molecular Biotechnology)
Schinkel, Helga (Fraunhofer Institute for Molecular Biology and Applied Ecology (IME))
Schillberg, Stefan (Fraunhofer Institute for Molecular Biology and Applied Ecology (IME))
Publication Information
Journal of Microbiology and Biotechnology / v.20, no.8, 2010 , pp. 1179-1184 More about this Journal
Abstract
We report the generation of the first monoclonal antibody that specifically binds to the polysaccharide chitosan. Mice were immunized with a mixture of chitosans, and hybridoma clones were screened for specific binders, resulting in the isolation of a single clone secreting a chitosan-specific IgM, mAbG7. In ELISAs, the antibody could bind to chitosans of varying composition, but demonstrated the highest affinity for chitosans with lower degrees of acetylation (DA) and very poor binding to chitin. We tested the ability of the antibody to bind to chitosan in situ, using preparations of fungal cell walls. Immunofluorescence microscopy confirmed that the antibody bound strongly to the cell walls of fungi with high levels of chitosan, whereas poor staining was observed in those species with cell walls of predominantly chitin or cellulose. The potential use of this antibody for the detection of fungal contamination and the protection of plants against fungal pathogens is discussed.
Keywords
Degree of acetylation (DA); D-glucosamine; IgM; N-acetyl-D-glucosamine; polysaccharides;
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