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http://dx.doi.org/10.4014/jmb.1001.01015

Characterization of Bacterial Structures in a Two-Stage Moving-Bed Biofilm Reactor (MBBR) During Nitrification of the Landfill Leachate  

Ciesielski, Slawomir (Department of Environmental Biotechnology, University of Warmia and Mazury in Olsztyn)
Kulikowska, Dorota (Department of Environmental Biotechnology, University of Warmia and Mazury in Olsztyn)
Kaczowka, Ewelina (Department of Environmental Biotechnology, University of Warmia and Mazury in Olsztyn)
Kowal, Przemyslaw (Department of Environmental Biotechnology, University of Warmia and Mazury in Olsztyn)
Publication Information
Journal of Microbiology and Biotechnology / v.20, no.7, 2010 , pp. 1140-1151 More about this Journal
Abstract
Differences in DNA banding patterns, obtained by ribosomal intergenic spacer analysis (RISA), and nitrification were followed in a moving-bed biofilm reactor (MBBR) receiving municipal landfill leachate. Complete nitrification (>99%) to nitrate was obtained in the two-stage MBBR system with an ammonium load of 1.09 g N-$NH_4/m^2{\cdot}d$. Increasing the ammonium load to 2.03 g N-$NH_4/m^2{\cdot}d$or more caused a decline in process efficiency to 70-86%. Moreover, at the highest ammonium load (3.76 g N-$NH_4/m^2{\cdot}d$), nitrite was the predominant product of nitrification. Community succession was evident in both compartments in response to changes in ammonium load. Nonmetric multidimensional scaling (NMDS) supported by similarity analysis (ANOSIM) showed that microbial biofilm communities differed between compartments. The microbial biofilm was composed mainly of ammonia-oxidizing bacteria (AOB), with Nitrosomonas europeae and N. eutropha being most abundant. These results suggest that high ammonium concentrations suit particular AOB strains.
Keywords
AOB; landfill leachate; nitrification; MBBR; RISA;
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1 Rowan, A. K., J. R. Snapeb, D. Fearnsidec, M. R. Barerd, T. P. Curtise, and I. M. Head. 2003. Composition and diversity of ammonia-oxidising bacterial communities in wastewater treatment reactors of different design treating identical wastewater. FEMS Microbiol. Ecol. 43: 195-206.   DOI   ScienceOn
2 Wyffels, S., P. Boeckx, K. Pynaert, W. Verstraete, and O. Van Cleemput. 2003. Sustained nitrite accumulation in a membrane-assisted bioreactor (MBR) for the treatment of ammonium-rich wastewater. J. Chem. Technol. Biotechnol. 78: 412-419.   DOI   ScienceOn
3 Gurtler, V. and V. A. Stanisich. 1996. New approaches to typing and identification of bacteria using the 16S-23S rDNA spacer region. Microbiology 142: 3-16.   DOI   ScienceOn
4 Kumar, S., K. Tamura, I. B. Jakobsen, and M. Nei. 2001. MEGA2: Molecular evolutionary genetics analysis software. Bioinformatics 17: 1244-1245.   DOI   ScienceOn
5 Surmacz-Gorska, J., K. Miksch, and M. Kita. 2000. Mo liwo ci podczyszczania odciekow z wysypisk metodami biologicznymi. Arch. Environ. Prot. 3: 43-54.
6 Otawa, K., R. Asano, Y. Ohba, T. Sasaki, E. Kawamura, F. Koyama, S. Nakamura, and Y. Nakai. 2006. Molecular analysis of ammonia-oxidizing bacteria community in intermittent aeration sequencing batch reactors used for animal wastewater treatment. Environ. Microbiol. 8: 1985-1996.   DOI   ScienceOn
7 Renou, S., J. G. Givaudan, S. Poulain, F. Dirassouyan, and P. Moulin. 2008. Landfill leachate treatment: Review and opportunity. J. Hazard. Mater. 150: 468-493.   DOI   ScienceOn
8 Shannon, C. E. and W. Weaver. 1963. The Mathematical Theory of Communication. University of Illinois Press, Urbana.
9 Tawfik, A., H. Temmink, G. Zeeman, and B. Klapwijk. 2006. Sewage treatment in a rotating biological contactor (RBC) system. Water Air Soil Pollut. 126: 193-206.
10 Qu, Y. Y., J. T. Zhou, J. Wang, L. L. Xing, N. Jiang, M. Gou, and M. Salah Uddin. 2009. Population dynamics in bioaugmented membrane bioreactor for treatment of bromoamine acid wastewater. Bioresour. Technol. 100: 244-248.   DOI   ScienceOn
11 Mota, C., M. A. Head, J. A. Ridenoure, J. J. Cheng, and F. L. de los Reyes. 2005. Effects of aeration cycles on nitrifying bacterial populations and nitrogen removal in intermittently aerated reactors. Appl. Environ. Microbiol. 71: 8565-8572.   DOI   ScienceOn
12 Muyzer, G., E. C. de Waal, and A. G. Uitterlinden. 1993. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes encoding for 16S rRNA. Appl. Environ. Microbiol. 59: 695-700.
13 Joo, S. H., D. J. Kim, I. K. Yoo, K. Park, and G. C. Cha. 2000. Partial nitrification in an upflow biological aerated filter by $O_2$ limitation. Biotechnol. Lett. 22: 937-940.   DOI   ScienceOn
14 Normand, P., C. Ponsonnet, X. Nesme, M. Neyra, and P. Simonet. 1996. ITS analysis of prokaryotes. In Akkermans, A. D. L., J. D. van Elsas, and F. J. de Bruijn (eds.). Molecular Microbial Ecology Manual Kluwer Academic Publishers, Dordrecht.
15 Odegaard, H., B. Rusten, and T. Westrum. 1994. A new moving bed reactor - applications and results. Water Sci. Technol. 29: 157-165.
16 Osborn, A. M., R. B. Moore, and K. N. Timmis. 2000. An evaluation of terminal-restriction fragment length polymorphism (T-RFLP) analysis for the study of microbial community structure and dynamics. Environ. Microbiol. 2: 39-50.   DOI   ScienceOn
17 Kalyuzhnyi, S. V. and M. A. Gladchenko. 2004. Sequenced anaerobic-aerobic treatment of high strength strong nitrogenous landfill leachate. Water Sci. Technol. 49: 301-308.
18 Konopka, A., M. Carrero-Colon, and C. H. Nakatsu. 2007. Community dynamics and heterogeneities in mixed bacterial communities subjected to nutrient periodicities. Environ. Microbiol. 9: 1584-1590.   DOI   ScienceOn
19 Kostman, J. R., T. D. Edlind, J. J. LiPuma, and T. L. Stull. 1992. Molecular epidemiology of Pseudomonas cepacia determined by polymerase chain reaction ribotyping. J. Clin. Microbiol. 30: 2084-2087.
20 Harms, G., A. Layton, H. Dionisi, I. Gregory, V. Garret, S. Hawkins, K. Robinson, and B. Sayler. 2003 Real-time PCR quantification of nitrifying bacteria in a municipal wastewater treatment plant. Environ. Sci. Technol. 37: 343-351.   DOI   ScienceOn
21 Hanaki, K., W. Chalermraj, and O. Sinichiro. 1990. Nitrification at low levels of dissolved oxygen with and without organic loading in a suspended-growth reactor. Water Res. 24: 297-302.   DOI   ScienceOn
22 Jensen, M. A., J. A. Webster, and N. Straus. 1993. Rapid identification of bacteria on the basis of polymerase chain reaction-amplified ribosomal DNA spacer polymorphisms. Appl. Environ. Microbiol. 59: 945-952.
23 Altschul, S. F., T. L. Madden, A. A. Schaffer, J. Zhang, Z. Zhang, W. Miller, and D. J. Lipman. 1997. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucleic Acids Res. 25: 3389-3402.   DOI
24 Chiemchaisri, C., W. Chiemchaisri, J. Junsod, S. Threedeach, and P. N. Wicranarachchi. 2009. Leachate treatment and greenhouse gas emission in subsurface horizontal flow constructed wetland. Bioresour. Technol. 100: 3808-3814.   DOI   ScienceOn
25 Yunxia, Z., Y. Xu, M. Jia, J. Zhou, S. Yuan, J. Zhang, and Z-P. Zhang. 2009. Stability of partial nitrification and microbial population dynamics in a bioaugmented membrane bioreactor. J. Microbiol. Biotechnol. 19: 1656-1664.   과학기술학회마을
26 Aakra, A., J. B. Utaker, and I. F. Nes. 1999. RFLP of rRNA genes and sequencing of the 16S-23S rDNA intergenic spacer region of ammonia-oxidizing bacteria: A phylogenetic approach. Int. J. Syst. Bacteriol. 49: 123-130.   DOI   ScienceOn
27 Thompson, J. D., D. G. Higgins, and T. J. Gibson. 1994. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22: 4673-4680.   DOI   ScienceOn
28 Tokutomi, T. 2004. Operation of a nitrite-type airlift reactor at low DO concentration. Water Sci. Technol. 49: 81-88.
29 Wang, R.-C., X.-H. Wen, and Q. Yi. 2005. Influence of carrier concentration on the performance and microbial characteristics of a suspended carrier biofilm reactor. Proc. Biochem. 40: 2992-3001.   DOI   ScienceOn
30 Yu, Z. and W. W. Mohn. 2001. Bacterial diversity and community structure in an aerated lagoon revealed by ribosomal intergenic spacer analyses and 16S ribosomal DNA sequencing. Appl. Environ. Microbiol. 67: 1565-1574.   DOI   ScienceOn
31 Zhang, B., B. Sun, M. Ji, and H. Liu. 2009. Population dynamic succession and quantification of ammonia-oxidizing bacteria in a membrane bioreactor treating municipal wastewater. J. Hazard. Mat. 165: 796-803.   DOI   ScienceOn
32 Gieseke, A., L. Bjerrum, M. Wagner, and R. Amann. 2003. Structure and activity of multiple nitrifying bacterial populations co-existing in a biofilm. Environ. Microbiol. 5: 355-369.   DOI   ScienceOn
33 Laitinen, N., A. Luonsi, and J. Vilen. 2006. Landfill leachate treatment with sequencing batch reactor and membrane bioreactor. Desalination 191: 86-91.   DOI   ScienceOn
34 Leblond-Bourget, N., H. Philippe, I. Mangin, and B. Decaris. 1996. 16S rRNA and 16S to 23S internal transcribed spacer sequence analyses reveal inter- and intraspecific Bifidobacterium phylogeny. Int. J. System. Bacteriol. 46: 102-111.   DOI   ScienceOn
35 Ganigue, R., H. Lopez, M. D. Balaguer, and J. Colprim. 2007. Partial ammonium oxidation to nitrite of high ammonium content urban landfill leachates. Water Res. 41: 3317-3326.   DOI   ScienceOn
36 Dionisi, H. M., A. C. Layton, G. Harms, I. R. Gregory, K. G. Robinson, and G. S. Sayler. 2002. Quantification of Nitrosomonas oligotropha-like ammonia-oxidizing bacteria and Nitrospira spp. from full-scale wastewater treatment plants by competitive PCR. Appl. Environ. Microbiol. 68: 245-253.   DOI   ScienceOn
37 Ciesielski, S., A. Cydzik-Kwiatkowska, and J. Turek. 2007. Molecular analysis of bacterial community diversity in sequencing batch reactor (SBR) operating in autotrophic conditions. Pol. J. Microbiol. 56: 45-51.
38 Clarke, K. R. 1993. Non-parametric multivariate analysis of changes in community structure. Aust. J. Ecol. 18: 117-143.   DOI
39 Clarke, K. R. and R. M. Warwick. 2001. Change in Marine Communities: An Approach to Statistical Analysis and Interpretation. Primer-E, Plymouth.
40 Du, Y., S. Chen, and D. Z. Sun. 2007. Short-cut nitrification of landfill leachate by aerobic moving-bed biofilm reactor. Environ. Sci. 28: 1039-1043.