Browse > Article
http://dx.doi.org/10.4014/jmb.0909.09027

Proteomic Analysis of Pancreata from Mini-Pigs Treated with Streptozotocin as Type I Diabetes Models  

Lee, Phil-Young (Medical Proteomics Research Center, KRIBB)
Park, Sung-Goo (Medical Proteomics Research Center, KRIBB)
Kim, Eun-Young (Medical Proteomics Research Center, KRIBB)
Lee, Myung-Sup (Medi Kinetics Co., Ltd.)
Chung, Sang-J. (BioNanotechnology Research Center, KRIBB)
Lee, Sang-Chul (Medical Proteomics Research Center, KRIBB)
Yu, Dae-Yeul (Disease Model Research Center, KRIBB,)
Bae, Kwang-Hee (Medical Proteomics Research Center, KRIBB)
Publication Information
Journal of Microbiology and Biotechnology / v.20, no.4, 2010 , pp. 817-820 More about this Journal
Abstract
Type 1 diabetes mellitus (T1DM) is an autoimmune disease characterized by extreme insulin deficiency due to an overall reduction in the mass of functional pancreatic ${\beta}$-cells. Several animal models have been used to study T1DM. Amongst these, the mini-pig seems to be the most ideal model for diabetes research, owing to similarities with humans in anatomy and physiology. The purpose of this study was to analyze differentially expressed pancreatic proteins in a streptozotocin (STZ)-induced mini-pig T1DM model. Pancreas proteins from mini-pigs treated with STZ were separated by two-dimensional gel electrophoresis, and 11 protein spots were found to be altered significantly when compared with control mini-pigs. The data in this study utilizing proteomic analysis provide a valuable resource for the further understanding of the T1DM pathomechanism.
Keywords
Mini-pig; proteomics; streptozotocin; type 1 diabetes mellitus;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 0  (Related Records In Web of Science)
연도 인용수 순위
1 Sparre, T., R. Bergholdt, J. Neryp, and F. Pociot. 2003. Application of genomics and proteomics in type 1 diabetes pathogenesis research. Expert Rev. Mol. Diagn. 3: 743-757.   DOI   ScienceOn
2 Sparre, R., M. R. Larsen, P. E. Heding, A. E. Karlsen, O. N. Jensen, and F. Pociot. 2005. Unraveling the pathogenesis of type 1 diabetes with proteomics. Mol. Cell. Proteomics 4: 441-457.   DOI   ScienceOn
3 Herr, R. R., J. K. Jahnke, and A. D. Argoudelis. 1967. The structure of streptozotocin. J. Am. Chem. Soc. 89: 4808-4809.   DOI
4 Chatzigeorgiou, A., A. Halapas, K. Kalafatakis, and E. Kamper. 2009. The use of animal model in the study of diabetes mellitus. In Vivo 23: 245-258.
5 Rossini, A. A., A. A. Like, W. L. Chick, M. C. Appel, and G. F. Cahill. 1977. Studies of streptozotocin-induced insulitis and diabetes. Proc. Natl. Acad. Sci. U.S.A. 74: 2485-2489.   DOI   ScienceOn
6 Skyler, J. S. 2007. Prediction and prevention of type 1 diabetes: Progress, problems, and prospects. Clin. Pharmacol. Ther. 81: 768-771.   DOI   ScienceOn
7 Nielsen, K., T. Sparre, M. R. Larsen, M. Nielsen, S. J. Fey, P. Mose Larsen, P. Roepstorff, J. Nerup, and A. E. Karlen. 2004. Protein expression changes in a $\beta$-cell maturation reflect an acquired sensitivity to IL-$1{\beta}$. Diabetologia 47: 62-74.   DOI   ScienceOn
8 Oyadomari, S., E. Araki, and M. Mori. 2002. Endoplasmic reticulum stress-mediated apoptosis in pancreatic $\beta$-cells. Apoptosis 7: 335-345.   DOI   ScienceOn
9 Marshall, M., U. Sprandel, and N. Zollner. 1975. Streptozotocin diabetes in a miniature pig. Res. Exp. Med. 165: 61-65.   DOI   ScienceOn
10 Marshall, M., H. Oberhofer, and J. Staubesand. 1980. Early micro- and macro-angiopathy in the streptozotocin diabetic minipig. Res. Exp. Med. 177: 145-158.   DOI   ScienceOn
11 Kang, T. H., K.-H. Bae, M.-J. Yu, W.-K. Kim, H.-R. Hwang, H. Jung, et al. 2007. Phosphoproteomic analysis of neuronal cell death by glutamate-induced oxidative stress. Proteomics 7: 2624-2635.   DOI   ScienceOn
12 Kubisch, C., M. D. Sans, T. Arumugam, S. A. Ernst, J. A. Williams, and C. D. Logsdon. 2006. Early activation of endoplasmic reticulum stress is associated with arginine-induced acute pancreatitis. Am. J. Physiol. Gastrointest. Liver Physiol. 291: G238-G245.   DOI   ScienceOn
13 Larsen, M. O. and B. Rolin. 2004. Use of the Gottingen minipig as a model of diabetes, with special focus on type 1 diabetes research. ILAR J. 45: 303-313.   DOI   ScienceOn
14 Kim, W. K., H. J. Cho, S. I. Ryu, H.-R. Hwang, D.-H. Kim, H. Y. Ryu, et al. 2008. Comparative proteomic analysis of peripheral blood mononuclear cells from atopic dermatitis patients and healthy donors. BMB Rep. 41: 597-603.   DOI   ScienceOn
15 Kim, W. K., H.-R. Hwang, D. H. Kim, P. Y. Lee, Y. J. In, H.-Y. Ryu, S. G. Park, K.-H. Bae, and S. C. Lee. 2008. Glycoproteomic analysis of plasma from patients with atopic dermatitis: CD5L and ApoE as potential biomarkers. Exp. Mol. Med. 40: 677-685.   DOI   ScienceOn
16 Canavan, J. P., P. A. Flecknell, J. P. New, K. G. Alberti, and P. D. Home. 1997. The effect of portal and peripheral insulin delivery on carbohydrate and lipid metabolism in a miniature pig model of human IDDM. Diabetologia 40: 1125-1134.   DOI   ScienceOn
17 Araki, E., S. Oyadomari, and M. Mori. 2003. Impact of endoplasmic reticulum stress pathway on pancreatic $\beta$-cells and diabetes mellitus. Exp. Biol. Med. 228: 1213-1217.
18 Atkinson, M. A. and R. Gianani. 2009. The pancreas in human type I diabetes: Providing new answers to age-old questions. Curr. Opin. Endocrinol. Obes. 16: 279-285.   DOI   ScienceOn
19 Kim, S. Y., P. Y. Lee, H.-J. Shin, D. H. Kim, S. Kang, H. B. Moon, et al. 2009. Proteomic analysis of liver tissue from HBx-transgenic mice at early stages of hepatocarcinogenesis. Proteomics 9: 5056-5066.   DOI   ScienceOn
20 Kim, S. W., H. J. Hwang, Y. M. Baek, S. H. Lee, H. S. Hwang, and J. W. Yun. 2008. Proteomic and transcriptomic analysis for streptozotocin-induced diabetic rat pancreas in response to fungal polysaccharide treatments. Proteomics 8: 2344-2361.   DOI   ScienceOn
21 Giarratana, N., G. Penna, and L. Adorini. 2007. Animal models of spontaneous autoimmune disease: Type 1 diabetes in the nonobese diabetic mouse. Methods Mol. Biol. 380: 285-311.   DOI   ScienceOn
22 Jin, J., J. Park, K. Kim, Y. Kang, S. G. Park, J. H. Kim, K. S. Park, H. Jun, and Y. Kim. 2009. Detection of differential proteomes of human $\beta$-cells during islet-like differentiation using iTRAQ labeling. J. Proteome Res. 8: 1393-1403.   DOI   ScienceOn
23 Jang, M., B. C. Park, S. Kang, S.-W. Chi, S. Cho, S. J. Chung, S. C. Lee, K.-H. Bae, and S. G. Park. 2009. Far upstream element-binding protein-1, a novel caspase substrate, acts as a cross-talker between apoptosis and the c-myc oncogene. Oncogene 28: 1529-1536.   DOI   ScienceOn
24 Xie, X., S. Li, S. Liu, Y. Lu, P. Shen, and J. Ji. 2008. Proteomic analysis of mouse islets after multiple low-dose streptozotocin injection. Biochim. Biophys. Acta 1784: 276-284.   DOI   ScienceOn
25 Yamamoto, H., Y. Uchigata, and H. Okamoto. 1981. Streptozotocin and alloxan induce DNA strand breaks and poly(ADP-ribose) synthetase in pancreatic islets. Nature 294: 284-286.   DOI   ScienceOn
26 Hu, L., S. Evers, Z. H. Lu, Y. Shen, and J. Chen. 2004. Two-dimensional protein database of human pancreas. Electrophoresis 25: 512-518.   DOI   ScienceOn