[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.4014/jmb.1002.02003

Isolation and Characterization of Psacotheasin, a Novel Knottin-Type Antimicrobial Peptide, from Psacothea hilaris

Hwang, Jae-Sam (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Lee, June-Young (School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University)
Hwang, Bo-Mi (School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University)
Nam, Sung-Hee (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Yun, Eun-Young (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Kim, Seong-Ryul (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Lee, Dong-Gun (School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University)

Publication Information

Journal of Microbiology and Biotechnology / v.20, no.4, 2010 , pp. 708-711 More about this Journal

Abstract

We report the isolation and characterization of a novel knottin-type antimicrobial peptide from the yellow-spotted long-horned beetle Psacothea hilaris. A cDNA encoding a 56-mer knottin-type propeptide was identified and its predicted molecular mass and pI were 5.92 kDa and 8.28, respectively. A 34-mer mature peptide was also selected and named herein as psacotheasin. The antimicrobial activity of chemically synthesized psacotheasin against human bacterial pathogens was subsequently investigated. The results showed that psacotheasin exerted potent activities against both Gram-positive and Gram-negative bacterial strains. The present study suggests that psacotheasin can be applied to develop novel therapeutic agents.

Keywords

Psacothea hilaris; psacotheasin; knottin-type peptide; antimicrobial peptide;

Citations & Related Records

Times Cited By KSCI : 4 (Citation Analysis)
Times Cited By Web Of Science : 6 (Related Records In Web of Science)

Reference
Cited By KSCI

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1	Membrane Perturbation Induced by Papiliocin Peptide, Derived from Papilio xuthus, in Candida albicans / [Lee, June-Young;Hwang, Jae-Sam;Hwang, Bo-Mi;Kim, Jin-Kyoung;Kim, Seong-Ryul;Kim, Yang-Mee;Lee, Dong-Gun;] / Journal of Microbiology and Biotechnology
2	The Novel Biological Action of Antimicrobial Peptides via Apoptosis Induction / [Cho, Jaeyong;Hwang, In-Sok;Choi, Hyemin;Hwang, Ji Hong;Hwang, Jae-Sam;Lee, Dong Gun;] / Journal of Microbiology and Biotechnology