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http://dx.doi.org/10.4014/jmb.0907.07026

Cultivation of Spirulina platensis Using Pig Wastewater in a Semi-Continuous Process  

Chaiklahan, Ratana (Pilot Plant Development and Training Institute, KMUTT)
Chirasuwan, Nattayaporn (Pilot Plant Development and Training Institute, KMUTT)
Siangdung, Wipawan (Pilot Plant Development and Training Institute, KMUTT)
Paithoonrangsarid, Kalyanee (BEC Unit, National Center for Genetic Engineering and Biotechnology, KMUTT)
Bunnag, Boosya (School of Bioresources and Technology, KMUTT)
Publication Information
Journal of Microbiology and Biotechnology / v.20, no.3, 2010 , pp. 609-614 More about this Journal
Abstract
The effluent from anaerobic digestion contains organic nitrogen and phosphorus, which are both required for growth of Spirulina platensis. Effluent (20%) from the upflow anaerobic sludge blanket (UASB) from a pig farm, supplemented with 4.5 g/l sodium bicarbonate ($NaHCO_3$) and 0.2 g/l urea fertilizer (46:0:0, N:P:K), was found to be not only a suitable medium for the growth of Spirulina platensis but also a low-cost alternative. Cost calculation showed that this medium is 4.4 times cheaper than modifized Zarrouk's medium. The average productivities of a semi-continuous culture grown under outdoor conditions in a 6-1 scale and a 100-1 pilot scale were 19.9 $g/m^2/d$ and 12 $g/m^2/d$, respectively. In addition, the biomass of organisms grown in UASB effluent contained approximately 57.9% protein, 1.12% $\gamma$-linolenic acid, and 19.5% phycocyanin. The average rates of bicarbonate, total nitrogen, and phosphorus removal were 380 mg/l/d, 34 mg/l/d, and 4 mg/l/d, respectively.
Keywords
Spirulina platensis; pig waste; nitrogen; phosphorus;
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