Browse > Article
http://dx.doi.org/10.4014/jmb.0908.08003

Differentiation of Three Lactobacillus rhamnosus Strains (E/N, Oxy, and Pen) by SDS-PAGE and Two-Dimensional Electrophoresis of Surface-Associated Proteins  

Jarocki, P. (Department of Biotechnology, Human Nutrition and Food Commodities, University of Life Science in Lublin)
Podlesny, M. (Department of Biotechnology, Human Nutrition and Food Commodities, University of Life Science in Lublin)
Wasko, A. (Department of Biotechnology, Human Nutrition and Food Commodities, University of Life Science in Lublin)
Siuda, A. (Department of Biotechnology, Human Nutrition and Food Commodities, University of Life Science in Lublin)
Targonski, Z. (Department of Biotechnology, Human Nutrition and Food Commodities, University of Life Science in Lublin)
Publication Information
Journal of Microbiology and Biotechnology / v.20, no.3, 2010 , pp. 558-562 More about this Journal
Abstract
SDS-PAGE of extracted surface-associated proteins of Lactobacillus rhamnosus strains E/N, Oxy, and Pen, was performed. The obtained protein patterns allowed differentiation of the examined strains, which was not accomplished by the commonly used RAPD genotypic method. The differentiation by the SDS-PAGE method proved to be a useful tool for strain-specific identification, which was further confirmed by 2DE analysis. Therefore, it can be used as an alternative or complementary method for both conventional and genotypic identification procedures, especially when closely related lactobacilli isolates are identified.
Keywords
Lactobacillus; polyacrylamide gel electrophoresis; random amplification of polymorphic DNA; surface-associated proteins;
Citations & Related Records

Times Cited By Web Of Science : 2  (Related Records In Web of Science)
연도 인용수 순위
  • Reference
1 Ma, D., P. Forsythe, and J. Bienenstock. 2004. Live Lactobacillus reuteri is essential for the inhibitory effect on tumor necrosis factor alpha-induced interleukin-8 expression. Infect. Immun. 72: 5308-5314.   DOI   ScienceOn
2 Wiatrzyk, A., U. Czajka, and B. Buholc. 2007. Resistance to antibiotics of Lactobacillus and Bifidobacterium strains present in probiotics preparations. Ped. Pol. 82: 910-915.   DOI   ScienceOn
3 Gu, R., Z. Yang, J. Cai, Z. Li, S. Chen, and Z. Luo. 2008. Analysis of different Lactobacillus rhamnosus by random amplified polymorphic DNA. Acta Microbiol. Sin. 48: 426-431.
4 Hebert, E. M., R. R. Raya, and G. S. de Giori. 2000. Use of SDS-PAGE of cell-wall proteins for rapid differentiation of Lactobacillus delbrueckii subsp. lactis and Lactobacillus helveticus. Biotechnol. Lett. 22: 1003-1006.   DOI   ScienceOn
5 Ruszczy ski, M., A. Radzikowski, and H. Szajewska. 2008. Clinical trial: Effectiveness of Lactobacillus rhamnosus (strains E/N, Oxy and Pen) in the prevention of antibiotic-associated diarrhoea in children. Aliment. Pharm. Therap. 28: 154-161.   DOI   ScienceOn
6 Sanchez, I., S. Sesena, and L. L. Palop. 2004. Polyphasic study of the genetic diversity of lactobacilli associated with "Almagro" eggplants spontaneous fermentation, based on combined numerical analysis of randomly amplified polymorphic DNA and pulsedfield electrophoresis patterns. J. Appl. Microbiol. 97: 446-458.   DOI   ScienceOn
7 Singh, S., P. Goswami, R. Singh, and K. J. Heller. 2009. Application of molecular identification tools for Lactobacillus, with a focus on discrimination between closely related species: A review. LWT Food Sci. Technol. 42: 448-457.   DOI   ScienceOn
8 Wright, A., R. Wait, S. Begum, B. Crossett, J. Nagy, K. Brown, and N. Fairweather. 2005. Proteomic analysis of cell surface proteins from Clostridium difficile. Proteomics 5: 2443-2452.   DOI   ScienceOn
9 Stiles, N. E. 1996. Biopreservation by lactic acid bacteria. Antonie van Leeuwenhoek 70: 331-345.   DOI
10 Tilsala-Timisjarvi, A. and T. Alatossava. 1998. Strain-specific identification of probiotic Lactobacillus rhamnosus with randomly amplified polymorphic DNA-derived PCR primers. Appl. Environ. Microbiol. 64: 4816-4819.
11 Rodtong, S. and G. W. Tannock. 1993. Differentiation of Lactobacillus strains by ribotyping. Appl. Environ. Microbiol. 59: 3480-3484.
12 Benito, M. J., M. J. Serradilla, S. Ruiz-Moyano, A. Martin, F. Perez-Nevado, and M. G. Cordoba. 2008. Rapid differentiation of lactic acid bacteria from autochthonous fermentation of Iberian dry-fermented sausages. Meat Sci. 80: 656-661.   DOI   ScienceOn
13 Goderska, K., M. Czarnecka, and Z. Czarnecki. 2002. Survival rate of chosen Lactobacillus bacteria type in media of different pH. EJPAU Food Sci. Technol. 5: 1-9.
14 Varmanen, P., T. Rantanen, A. Palva, and S. Tynkkynen. 1998. Cloning and characterization of a prolinase gene (pepR) from Lactobacillus rhamnosus. Appl. Environ. Microbiol. 64: 1831-1836.
15 Nguyen, T. D. T., J. H. Kang, and M. S. Lee. 2007. Characterization of Lactobacillus plantarum PH04 a potential probiotic bacterium with cholesterol-lowering effects. Int. J. Food. Microbiol. 113: 358-361.   DOI   ScienceOn
16 Axelsson, L. 2004. Lactic acid bacteria: Classification and physiology, pp. 1-66. In S. Salminen, A. von Wright, and A. Ouwehand (eds.). Lactic Acid Bacteria. Microbiological and Functional Aspects, 3rd Ed. Marcel Dekker, New York.
17 Alander, M., R. Satokari, R. Korpela, M. Saxellin, T. Vilpponen-Salmela, T. Mattila-Sandholm, and A. von Wright. 1999. Persistence of colonization of human colonic mucosa by a probiotic strain, Lactobacillus rhamnosus GG, after oral consumption. Appl. Environ. Microbiol. 65: 351-354.
18 Ouwehand, A. C., S. Salminen, and E. Isolauri. 2002. Probiotics: An overview of beneficial effects. Antonie van Leeuwenhoek 82: 279-289.   DOI   ScienceOn
19 Pot, B., P. Vandamme, and K. Kersters. 1994. Analysis of electrophoretic whole-organism protein fingerprints, pp. 493-521. In M. Goodfellow and A. G. O'Donnel (eds.). Chemical Methods in Prokaryotic Systematics. Wiley, Chichester, U.K.
20 Nigatu, A., S. Ahrne, and G. Molin. 2001. Randomly amplified polymorphic DNA (RAPD) profiles for the distinction of Lactobacillus species. Antonie van Leeuwenhoek 79: 1-6.   DOI   ScienceOn
21 Sanchez, B., P. Bressollier, S. Chaignepain, J. M. Schmitter, and M. C. Urdaci. 2008. Identification of surface-associated proteins in the probiotic bacterium Lactobacillus rhamnosus GG. Int. Dairy J. 19: 85-88.
22 Kwon, O. S. 2000. Characterization of isolated Lactobacillus spp. and classification by RAPD-PCR analysis. J. Microbiol. 38: 137-144.
23 Tynkkynen, S., R. Satokari, M. Saarela, T. Mattila-Sandholm, and M. Saxelin. 1999. Comparison of ribotyping, randomly amplified polymorphic DNA analysis, and pulsed-field gel electrophoresis in typing of Lactobacillus rhamnosus and L. casei strains. Appl. Environ. Microbiol. 65: 3908-3914.
24 Gatti, M., M. E. Fornasari, and E. Neviani. 2001. Differentiation of Lactobacillus delbrueckii subsp. bulgaricus and Lactobacillus delbrueckii subsp. lactis by SDS-PAGE of cell-wall proteins. Lett. Appl. Microbiol. 32: 352-356.   DOI   ScienceOn