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http://dx.doi.org/10.4014/jmb.0904.04007

Omics-Based Analysis of the luxS Mutation in a Clinical Isolate of Escherichia coli O157:H7 in Korea  

Kim, Jong-Chul (Division of Enteric Bacterial Infections, Center for Infectious Diseases, National Institute of Health)
Yoon, Jang-Won (Advanced Human Resource and Research Group for Medical Science (BK21), Konkuk University School of Medicine)
Kim, Jong-Bae (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University)
Oh, Kyung-Hwan (Division of Enteric Bacterial Infections, Center for Infectious Diseases, National Institute of Health)
Park, Mi-Sun (Division of Enteric Bacterial Infections, Center for Infectious Diseases, National Institute of Health)
Lee, Bok-Kwon (Division of Enteric Bacterial Infections, Center for Infectious Diseases, National Institute of Health)
Cho, Seung-Hak (Division of Enteric Bacterial Infections, Center for Infectious Diseases, National Institute of Health)
Publication Information
Journal of Microbiology and Biotechnology / v.20, no.2, 2010 , pp. 415-424 More about this Journal
Abstract
The purpose of this study was to investigate the relationship between the global regulatory mechanism known as quorum sensing and expression of virulence factors in Escherichia coli O157:87. A nonpolar luxS deletion was introduced into the chromosome of strain CI03J, a human clinical isolate from South Korea, to create the ${\Delta}luxS$ mutant strain ML03J. Phenotypic characterization of wild-type and mutant strains demonstrated that ML03J had no obvious growth or metabolic defects on 0.2% glucose LB medium, produced a functionally defective flagellum, and could not utilize sorbose; the biological significance of sorbose utilization is unknown. Omics-based analysis revealed the involvement of LuxS in the transcriptional activation of several flagella/chemotaxisrelated genes (flhD; fliA, C, D, S, Z; and cheA, Y, Z), repression of glutamate-dependent acid resistance genes (gadAB), and expression of virulence factors including Shiga toxin, hemolysin, and SepD within the LEE pathogenicity island.
Keywords
Escherichia coli O157:H7; clinical isolates; Korea; luxS mutation; omics;
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